This research aimed to determine the potency of two Trichoderma harzianum isolates in solid and liquid organic formulas to control bacterial wilt and on growth and yield of tomato in the field. This research was carried out at the Laboratory of Plant Protection and the tomato field at Banteran Village, Sumbang Sub-District, Banyumas Regency at altitude of 600 m above sea level for four months. A randomized block design was used with five replicates. The treatments were control, T. harzianum T10, T. harzianum T215, combination of T. harzianum T10 + T. harzianum T215, and bactericide (a.i. 20% streptomycin sulfate). Variables observed were pathosystem components (incubation period, infection rate, disease intensity, and late populations of the antagonists), growth components (plant height, plant fresh and dry weight, and root fresh and dry weight), yield components (number of fruits, fruit weight), and phenolic compounds analysis qualitatively. The results showed that T. harzianum T10 + T. harzianum T215 was effective to suppress the disease as 58.61%. The treatment of T. harzianum T10 + T. harzianum T215 was effective to increase crop height, fresh weight of plants, dry weight of plants, fresh weight of roots, dry weight of roots, number of fruits and fruit weight as 38.86, 35.37, 51.67, 24.78, 37.41, 40.61, and 53.22%, respectively. All treatments could increase phenolic compound content qualitatively.

Wereng batang coklat adalah hama utama padi yang belum dapat diatasi hingga kini dan tidak dapat dikendalikan dengan insektisida kimia bahkan dapat menimbulkan dampak negatif. Oleh karenanya, diperlukan pengendalian ramah lingkungan dan aman. Penelitian bertujuan untuk menentukan pengaruh aplikasi metabolit sekunder jamur entomopatogen terhadap wereng batang coklat pada padi varietas Ciliwung in planta. Penelitian dilaksanakan di screen house dan Laboratorium Perlindungan Tanaman, Fakultas Pertanian, Universitas Jenderal Soedirman, Purwokerto selama 5 bulan. Rancangan Acak Kelompok non-faktorial dengan 6 perlakuan dan 4 ulangan digunakan, dengan perlakuan adalah kontrol, metabolit sekunder Beauveria bassiana B10, Beauveria bassiana B16, Metarhizium anisopliae, Lecanicillium lecani, dan insektisida bahan aktif imidakloprid. Variabel yang diamati adalah mortalitas, waktu kematian, intensitas serangan, dan tinggi tanaman padi. Hasil penelitian menunjukkan bahwa aplikasi metabolit sekunder B. bassiana B16 paling efektif mengendalikan N. lugens, yang ditunjukkan oleh kematian wereng batang coklat paling cepat yaitu 6,57 hari setelah aplikasi, dengan mortalitas 52%, dan mampu menekan intensitas serangan N. lugens paling rendah yaitu 6,48%. Metabolit sekunder jamur entomopatogen tidak berpengaruh terhadap tinggi tanaman.

The information system is one of the strategic means in patient care-oriented management. So far, Regional Hospital RAA Soewondo Pati has never conducted an evaluation related to the management information system (HMIS), so it is not known that the existing system is effective and efficient to answer patient needs. The purpose of this study was to determine the effect of the hospital management information system with the structural equation modeling method approach. This research is a case study research with data collection method using a questionnaire. The data that has been collected is then analyzed using SEM analysis. The results of his research stated that the quality of the system had a significant positive effect on the use of information systems. There is a significant effect of system quality on user satisfaction. There is a significant positive effect between the quality of information on the intensity of the use of information. In this study, the intensity of the use of information systems does not play a role in increasing user satisfaction in using information systems. User satisfaction has a significant positive effect on net benefits.

Abstract. Soesanto L, Saputra DA, Sastyawan MWR, Mugiastuti E, Suprapto A, Rahayuniati RF. 2023. Secondary metabolites of the granular form of Pseudomonas fluorescens P60 and its applications to control tomato bacterial wilt. Biodiversitas 24: 2475-2482. Bacterial wilt is one of the important diseases of tomatoes caused by Ralstonia solanacearum (Smith 1896) Yabuuchi et al. 1996. Its control can be achieved by applying secondary metabolites of Pseudomonas fluorescens Migula 1895 P60 in granular form. The study aimed to determine the shelf life of the secondary metabolites P. fluorescens P60' in granular form and its application to manage bacterial wilt and the performance of tomatoes. The study was conducted for five months at the Plant Protection Laboratory and Experimental Farm, Faculty of Agriculture, Universitas Jenderal Soedirman. A randomized block design was used with four replicates and six treatments, consisting of R. solanacearum (control), R. solanacearum + 1, 5, 10, or 15 g, and bactericide (a.i. agrimycin sulphate 20%). Variables observed were inhibition zone, incubation period, disease intensity, the area under disease progress curve (AUDPC), crop height, crop fresh and dry weight, root fresh and dry weight, and phenolic compounds qualitatively. Results of the research showed that the granular formula until the 4th week still had an inhibition zone between 21.67-23.34%. However, the granules stored for as much as 15 g for four weeks effectively reduced disease intensity by 75.00%, decreased AUDPC value by 74.72%, and increased plant height, root dry weight, and fresh crop weight as well 44, 62.5, and 65.65 %, respectively. In addition, the granular formula increased the content of tomato phenolic compounds qualitatively.

This research aimed to determine the best liquid media for the propagation of weed pathogenic fungi, the duration of the fungus storage on the media, and their virulence on goatweed (Ageratum conyzoides). The research consisted of two stages, i.e., the propagation of weed pathogenic fungi in alternative liquid media using a factorial completely randomized design, with the first factor being the pathogenic weed fungus (Curvularia sp., Fusarium sp., and Chaetomium sp.) and the second one being the media (tempeh or tapioca liquid waste) with four replicates. Applications were carried out using a hand sprayer on the underside of weed leaves at a density of 106 conidia or cfu mL-1. Each weed was sprayed with 10 mL of the solution. Variables observed were conidia density, number of colonies, incubation period and symptoms, disease intensity, the area under the disease progress curve (AUDPC), plant height, number of leaves, dry crown weight, and dry root weight. The results showed that tempeh and tapioca liquid waste media could be used as alternative media for multiplying pathogenic fungi. The conidia density of Curvularia sp. was 2.375 × 109 conidia mL-1 higher than that of Fusarium sp. at 1.7 × 109 conidia mL-1 and Chaetomium sp. at 9.5×104 cfu mL-1. Curvularia sp. propagated in tempeh liquid waste was able to cause damage to the leaves of goatweed as shown successively from the incubation period of 3.33 dai or accelerating 81.50%, increasing the disease intensity of 88.78%, and the AUDPC of 713.25% days compared to control. The most effective shelf life of Curvularia sp., Fusarium sp., and Chaetomium sp. in both tempeh and tapioca liquid waste media was found at six weeks at room temperature. Curvularia sp. in tapioca liquid waste could decrease weed height, the number of leaves, shoot dry weight, and root dry weight by 45.11, 28.65, 22.12, and 46.25%, respectively, compared to control.