Objective: The objective of the study was to evaluate the genotoxic and mutagenic potentials of Kalanchoe pinnata alcoholic leaves extract.Methods: Single cell gel electrophoresis (COMET assay) was adopted to investigate genotoxicity potential of standardized ethanolic leaves extract at the concentration of 10, 50, and 100 µg/ml, respectively, using human lymphocytes.Results: K. pinnata leaves extract did not completely damage DNA in human lymphocytes but low, medium, and high-level damage was found in with positive control. The DNA damage level decreases in low, medium, and high (12±1, 6±1, and 1±1) without positive control at the concentration of 100 µg/ml. Conclusion:It was concluded that the parent material did not induce mutagenic and genotoxic potentials at the concentrations 10, 50, and 100 µg/ml but its metabolites are inducing genotoxicity in various models.