Objective:- Diabetic foot ulcers, the aim of the study for determine some types of microorganisms from diabetic foot ulcers thatcauses inflammation and long-term complications and activity study of ZnO NPs biosynthesis by Aspergillus niger against isolatedbacteria, collected from infected patients at general Al -Hashimiya hospital in Babylon Governorate.Methods:-collected 100 swabs, cultured it, bacteria was diagnostic by morphological and microscopic, standard biochemical tests andVitec,the isolates identified by molecular diagnosis by extraction of DNA,using specific universal primer 16SrRNA (1239bp) andpolymerase chain reaction (PCR), study biosynthesis,characterization of zinc Oxide Nanoparticles to inhibited bacterial growth.Results:-The results were (26 aerobic bacterial isolates positive) present such as Gram positive Staph aureus , as regards the aerobicGram negative bacilli, Klepsiella pneumoniae, Proteus mirabilis, Escherichia coli, The zinc oxide nanoparticles created byAspergillus niger were spherical in shape with size 13.5nm that used as antibacterial.Discussion:-Through determination of MIC were concentrations of ZnO NPs (31.25, 62.5μg /ml, 125μg/mL, 250μg/ml, 500μg /ml),(31.25, 62.5μg /ml) not prevent growth and not inhibition while 125μg/mL determine as MIC, two best concentrations are 250μg/ml,500μg /ml both give inhibition zones.conclusion:- Gram negative bacteria was predominantly for diabetic foot ulcers, Aspergillus niger was shown to be a stabilizercapable of producing spherical ZnO nanoparticles, due to is very suitable candidate to carry out antimicrobial operation, as a result toits small size.
