BackgroundLeptospirosis is one of the most widespread zoonotic diseases, which is of global medical and veterinary importance, and also a re-emerging infectious disease. The main tracks of transmission are known; however, the relative importance of each of the components and the respective environmental risk factors are unclear. We aimed to assess and specify quantitative evidence of environmental risks of leptospirosis transmission.Methods/findingsA database of pre-selected studies, with publication dates from 1970 until 2008, was provided by an expert group. The database has been updated until 2015 using a text mining algorithm. Study selection was based on stringent quality criteria. A descriptive data analysis was performed to calculate the medians of the log transformed odds ratios. From a selection of 2723 unique publications containing information on leptospirosis, 428 papers dealing with risk factors were identified. Of these, 53 fulfilled the quality criteria, allowing us to identify trends in different geo-climatic regions. Water associated exposures were, with few exceptions, associated with an increased leptospirosis risk. In resource poor countries, floods and rainfall were of particular importance, whereas recreational water activities were more relevant in developed countries. Rodents were associated with increased leptospirosis risk, but the variation among studies was high, which might be partly explained by differences in exposure definition. Livestock contact was commonly associated with increased risk; however, several studies found no association. The median odds ratios associated with dog and cat contacts were close to unity. Sanitation and behavioural risk factors were almost always strongly associated with leptospirosis, although their impact was rarely investigated in Europe or North America.ConclusionThis review confirms the complex environmental transmission pathways of leptospirosis, as previously established. Although, floods appeared to be among the most important drivers on islands and in Asia, the consistent pattern observed for exposure to rodents and behavioural and sanitation related risk factors indicate potential areas for intervention.

Background: In sub-Saharan Africa, the contribution of nontuberculous mycobacteria (NTM) to the aetiology of TB-like disease is uncertain. The aim of this pilot study was to generate data regarding the clinical relevance of pulmonary NTM to respiratory disease in the population making use of the platform provided by a nationwide TB prevalence survey.Methods & Materials: Sputa from eligible participants who had abnormal chest X-ray and/or symptoms suggestive of pulmonary tuberculosis (PTB) were cultured using the MGIT 960 system. DNA was extracted from all culture positive isolates confirmed not to be M. tuberculosiscomplex using the BD MGIT TBc identification test. These isolates were further characterized using 16S rRNA gene sequence analysis.Results: 16S rRNA gene sequence analysis was performed on DNA isolated from suspected NTM cultures from 57 patients. Eight (14.8%) patients had NTM isolated from their sputa. (37.5%) of these patients had M. kansasii and 62.5% had M. avium complex. One patient with M. kansasii was sputum smear positive and met the stringent American Thoracic Society (ATS) criteria for NTM lung disease.Conclusion: 16S rRNA gene sequence analysis was performed on DNA isolated from suspected NTM cultures from 57 patients. Eight (14.8%) patients had NTM isolated from their sputa. (37.5%) of these patients had M. kansasii and 62.5% had M. avium complex. One patient with M. kansasii was sputum smear positive and met the stringent American Thoracic Society (ATS) criteria for NTM lung disease.