Applying laser technology to the growth of plants to limit the use of chemical fertilizers is an interesting topic in agriculture. The main idea is to preserve the environment, ensuring product quality while still achieving high productivity, we decided to carry out this research project, to investigate the effect of the low-level laser (the wavelengths 532nm, 850nm, and 940nm) on stems and leaf development. It is expected that with these research results, the implementation method will be widely disseminated in the high agricultural sector, coming closer to farmers. Moreover, the results of the analysis of the composition of bitter melon stems and leaves will be applied in medical treatment (such as diabetes, wound treatment, anti-oxidation, anti-bacteria ...)