Background: Graphene oxide (GO) has drawn much attention as excellent platform to which silver nanoparticles (AgNPs) can be anchored for the production of biomedical nanocomposites. Yet, the potential toxicity of GO-AgNPs nanocomposites to animal and human is complex to evaluate and remains largely unknown. Results: Our data indicated that GO-AgNPs caused cytotoxicity in dose-dependent manner. GO-AgNPs induced significant cytotoxicity by the loss of cell viability, production of reactive oxygen species (ROS), cell cycle arrest, increasing leakage of lactate dehydrogenase (LDH) and level of Malondialdehyde (MDA), increasing expression of pro-apoptotic genes and decreasing expression of anti-apoptotic genes. Conclusions: Taken together, our study demonstrated that GO-AgNPs potentially induce oxidative damage to DNA, which result in toxicity and cell apoptosis in caprine fetal fibroblast cell due to an increased generation of ROS. It strongly suggests that applications of GO-AgNPs nanocomposite in animal must be further evaluated.

Background: Graphene oxide (GO) has drawn much attention as excellent platform to which silver nanoparticles (AgNPs) can be anchored for the production of biomedical nanocomposites. Yet, the potential toxicity of reduced graphene oxide- silver nanoparticles (GO-AgNPs) nanocomposites to animal and human is complex to evaluate and remains largely unknown.Results: Our data indicated that GO-AgNPs caused cytotoxicity in dose-dependent manner. GO-AgNPs induced significant cytotoxicity by the loss of cell viability, over-production of reactive oxygen species (ROS), increased leakage of lactate dehydrogenase (LDH) and level of malondialdehyde (MDA), increased expression of pro-apoptotic genes and decreased expression of anti-apoptotic genes.Conclusions: This study demonstrated that GO-AgNPs potentially induced oxidative stress, which resulted in toxicity and cell apoptosis in caprine fetal fibroblast cell due to an increased generation of ROS. For antibacterial applications of GO-AgNPs nanocomposite in animal, the toxical effect must be further evaluated.