A total of 125 random samples of raw milk and soft cheese (25 each) of cow's milk, sheep's milk, goat's milk, kareish cheese and Domiati cheese samples were collected from different markets and shops in Alexandria city, Egypt and examined for the presence of Staphylococcus aureus and Streptococcus agalactiae as food poisoning and mastitis causing organisms. The incidence of Staphylococcus aureus in examined samples were 28, 36, 40, 20 and 16% in the examined cow's milk, sheep's milk, goat's milk, kareish cheese and Domiati cheese, respectively. Streptococcus agalactiae was detected in 16, 20, 24, 12 and 4% of the examined samples, respectively. Streptococcus agalactiae was identified using primers V1 and V2, specific to rRNA as an early diagnosis of subclinical mastitis using, PCR technique. The sanitary and public health importance of these organisms as well as control measures to improve the quality of dairy products and to safeguard the consumers from infection were discussed.

A total of 200 samples (100 Farmer raw cow's milk, 60 market milk and 40 fresh cream) were collected from individual farmers, different dairy shops and milk separation centers distributed through Beni-Seuf and El-Minia cities, Egypt. The milk samples were subjected to serological test for detection of Brucella antibodies, using Milk ring test (MRT), Whey Buffered acidified plate antigen test (wBAPAT) and Whey Rose Bengal plate test (wRBPT). Out of 100 farmers milk samples examined by MRT. 30%, 7% and 63% were positive, suspicious and negative respectively. Also, 44% and 42% of these samples were positive for wBAPAT and wRBPT, respectively. On the other hand, Out of 60 market milk samples examined by MRT. 23.3%, 8.4% and 68.3% were positive, suspicious and negative, respectively, while 53.4% and 48.4% were positive for wBAPAT and wRBPT, respectively. All samples were examined bacteriologically for presence of Brucella organisms. The prevalence of Brucella species in farmers milk, market milk and cream samples were 28%, 60% and 32.5%, respectively. All isolates were typed as Brucella melitensis biovar 3. The public health significance and suggestive control measures were discussed.