Antioxidant is an interesting bioactivity since it has several correlations with disease such as Alzheimer, cancer, ageing and many others in which were promoted by free radicals. Investigation on our endophytic fungus collection namely Fennelia nivea NRRL 5504 revealed that the fermented mediumhas antioxidant property against DPPH through free radical scavenging effect. Fermentation in liquid medium i.e. Potatoes Dextrose Broth, resulted that the fermented medium reached about 86.51% (p < 0.05) of free radical inhibition on the eighth day. Results ofphytochemical screening from extracted fermentation medium showed the presence of terpenes, triterpenes, phenolic compound, tannin, flavonoid and also saponin. The highest antioxidant activity was showed by ethyl acetate extract. Calculation on total phenolic content of ethyl acetate crude extract was 0.544 mg g(-1) equivalent to pyrogallol. By exposure above, we have alternative source of antioxidant that came from our endophytic fungus collection which we isolated previously from Typhonium divaricatum Lodd.