Background: Physalis angulata L. or Cutleaf groundcherry is underutilized crop that has not been widely cultivated and is often considered weeds. Cutleaf groundcherry is rich in nutritional and phytochemical contents, which is beneficial for human health. Information on the growth, yield and quality content of cutleaf groundcherry lines is essential for future development. The objective of this experiment was to determine the variation among the lines of cutleaf groundcherry in growth, yield and fruits quality. Methods: A greenhouse study was conducted in August to December, 2021. Nine lines of cutleaf groundcherry were tested in randomized block design by following the recommended agronomic practcies. Result: The results show that nine lines of cutleaf groundcherry were varied with significant differences in growth, yield and quality characteristics. The line PA-06 was superior to other lines in vegetative growth and total dry weight, yield characteristics, namely fruit weight fruit-1, fruit diameter and fruit weight plant-1. The nine lines had the same as total soluble solids and ascorbic acid, but differences in β-carotene and antioxidant.