A retrospective study of the experiments performed during the past 15 years on infections of Galba truncatula with Fasciola hepatica was carried out to determine what susceptible populations of snails might be used for the commercial production of metacercariae, and to examine this metacercarial production in relation to the characteristics of snail infections. Of the four groups of snail populations studied, the ablest snails to sustain a complete larval development of F. hepatica originated from populations living on siliceous soils at 600 m and more in altitude. In contrast, snail populations living along river banks on siliceous soils were inappropriate due to the poor characteristics of snail infections (high snail mortality, low prevalence of snail infections, and low number of cercariae produced). Except for these last populations, 86-87% of cercaria-shedding (CS) snails in the other populations shed less than 300 cercariae, even if a maximum of 1,772 cercariae were obtained from a single snail. The date of the first cercarial shedding at 20 degrees C began during week 7 or 8 PE for 80.1-83.5% of CS snails. Most metacercariae (82.0-85.9% of the total production) were recorded during the first 10 days of the patent period. In these conditions, the authors collected metacercarial production up to the beginning of week 10 PE (20 degrees C) and did not use snails that shed their cercariae during the following weeks due to too low numbers of parasites. This method allows to have a continuous production of metacercariae over time by using successive groups of infected snails, each being separated from the other by a fortnight's time.

A retrospective study on a total of 669 Galba truncatula (three groups) experimentally infected with Fasciola hepatica was carried out to determine why 6- to 8-day interwave intervals, separating the successive waves of cercarial shedding, occurred with a regular pattern in some snails during the whole patent period. In the three groups of snails, the number of cercariae per shedding wave peaked at the second wave and subsequently decreased up to the fifth wave. The mean length of interwave intervals ranged from 6.8 to 7.8 days and only showed insignificant variations. The number of free cercariae recorded at the end of each interwave interval significantly decreased over the patent period. Similar findings were also noted for intraredial cercariae in the first redial generation and the first cohort of the second generation. By contrast, the number of intraredial cercariae significantly increased along the patent period from the second interwave interval. In the case of each interval separately considered, most numerical variations noted for free cercariae or for intraredial cercariae were insignificant. The periodicity of 6.8-7.8 days found for interwave intervals in the present study might correspond to the infradian-type rhythm already reported for the cercarial shedding of F. hepatica. However, snails showing such regular pattern in cercarial shedding along the patent period were few in number, and one may wonder about the reasons of such snails in the case of F. hepatica.