Field experiments were conducted during 2013-14 and 2014-15 to evaluate the new insecticides for the management of mite, Polyphagotarsonemus latus banks and dissipation of effective insecticide on capsicum. Among the seven insecticides, mean of two seasons under poly house condition, population was less with spiromesifen (0.06 mites/ leaf) followed by diafenthiuron (2.21 mites/ leaf), triazophos (3.68 mites/ leaf) and thiamethoxam (5.30 mites/ leaf) which were significantly superior over untreated check (11.33). Spiromesifen residues were quantified through regular sampling till the residues are below determination level (BDL) of 0.05 mg kg -1 following the validated QuEChERS method. The qualitative and quantitative analysis of spiromesifen was performed on LC-MS/MS (PDA). Initial deposits of 1.61 mg kg -1 of spiromesifen detected at 2 hours after last spray, dissipated to BDL at 10 th day after spray. The half-life and safe waiting period for harvest was 2.09 and 10.00 days, respectively.
Plant growth promoting bacteria enhance the growth in plants by solubilizing insoluble minerals, producing phytohormones and by secreting enzymes that resist pathogen attack. The present study was aimed at identifying the potential of Lysinibacillus macroides isolated from pea plant possessing rich microbial rhizobiome diversity in promoting the growth of tomato plant (Solanum lycopersicum L). Potential of L. macroides in the promotion of S. lycopersicum L. growth by increased shoot length, terminal leaf length and breadth was assessed. Anatomical sectioning of stem and root revealed no varied cellular pattern indicating that the supplemented bioculture is not toxic to S. lycopersicum. Plantlets treated with L. macroides along with organic compost showed an increased total phenol content (17.58±0.4 mg/g) compared to control samples (12.44±0.41 mg/g). Carbohydrate content was noticed to be around 1.3 folds higher in the L. macroides plus compost mixture supplemented slots compared to control sample. Significant increase in shoot length was evident in the L. macroides plus compost supplied slots (23.4±2.7 cm). Plant growth promoting properties might be due to the nitrogen fixing activity of the bacteria which enrich the soil composition along with the nutrients supplied by the organic compost. Rich microbial rhizobiome diversity in pea plant and the usage of L. macroides from a non-conventional source improves the diversity of the available PGPR for agricultural practices. Further research is needed to detect the mechanism of growth promotion and to explore the plant microbe interaction pathway.
:Chlorantraniliprole is a broad spectrum foliar insecticide with contact and systemic action, widely used on vegetables in India for the management of lepidopteran insects both in field and poly houses. Chlorantraniliprole is not registered for use on capsicum in India and hence, maximum residue limits are not available as per Food Safety and Standards Authority of India. However, use of chlorantraniliprole in open field and poly house is very common hence, chlorantraniliprole residues are found in survey samples. A research project was taken to study dissipation pattern of chlorantraniliprole 20 SC in both open fields and poly houses, when applied thrice @ 60 g a.i.ha -1 , first spray at fruit initiation followed by second and third spray at 10 days interval as per the farmers practice. Chlorantraniliprole residues were quantified through regular sampling till the residues are below determination level (BDL) of 0.05 mg kg -1 following the validated QuEChERS method. The qualitative and quantitative analysis of chlorantraniliprole was performed on LC-MS/MS (PDA). Initial deposits of 0.36 mg kg -1 were detected in capsicum samples collected from open filed, which dissipated to BDL in 7.0 days while in poly house, initial deposits of 1.31 mg kg -1 were dissipated to BDL in 15.0 days. The waiting period for safe harvest was worked out to be 7.0 and 15.0 days when chlorantraniliprole 20 SC @ 60 ml a.i. ha -1 sprayed thrice in open and poly house conditions, respectively. Dissipation is slow in poly house compared to open fields due to various factors. In both situations initial deposits are lower than the MRL (2 mg kg -1 ) of Codex Alimentarius Commission hence, a pre-harvest interval of 7.0 and 15.0 day is recommended.
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