This study aimed to evaluate the quality of the water in the Guebli River. Five sampling stations were selected along the river to determine the important physicochemical and biological parameters of the water. Our results show that water from the downstream station is highly polluted because it has high levels of most physicochemical parameters compared to other stations. This situation may be due to the accumulation of waste discharged by human activities along the river. The study also shows that the least polluted stations have a very varied faunal structure compared to polluted stations.