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This paper describes an interactive virtual laboratory for experimenting with an outdoor tubular photobioreactor (henceforth PBR for short). This virtual laboratory it makes possible to: (a) accurately reproduce the structure of a real plant (the PBR designed and built by the Department of Chemical Engineering of the University of Almería, Spain); (b) simulate a generic tubular PBR by changing the PBR geometry; (c) simulate the effects of changing different operating parameters such as the conditions of the culture (pH, biomass concentration, dissolved O2, inyected CO2, etc.); (d) simulate the PBR in its environmental context; it is possible to change the geographic location of the system or the solar irradiation profile; (e) apply different control strategies to adjust different variables such as the CO2 injection, culture circulation rate or culture temperature in order to maximize the biomass production; (f) simulate the harvesting. In this way, users can learn in an intuitive way how productivity is affected by any change in the design. It facilitates the learning of how to manipulate essential variables for microalgae growth to design an optimal PBR. The simulator has been developed with Easy Java Simulations, a freeware open-source tool developed in Java, specifically designed for the creation of interactive dynamic simulations.

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Control of wastewater treatment plants (WWTPs) is challenging not only because of their high nonlinearity but also because of important external perturbations. One the most relevant of these perturbations is weather. In fact, different weather conditions imply different inflow rates and substance (e.g., N-ammonia, which is among the most important) concentrations. Therefore, weather has traditionally been an important signal that operators take into account to tune WWTP control systems. This signal cannot be directly measured with traditional physical sensors. Nevertheless, machine learning-based soft-sensors can be used to predict non-observable measures by means of available data. In this paper, we present novel research about a new soft-sensor that predicts the current weather signal. This weather prediction differs from traditional weather forecasting since this soft-sensor predicts the weather conditions as an operator does when controling the WWTP. This prediction uses a model based on past WWTP influent states measured by only a few physical and widely applied sensors. The results are encouraging, as we obtained a good accuracy level for a relevant and very useful signal when applied to advanced WWTP control systems.

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