Present study was conducted to identify effective microsporogeneis and flower bud stage for application of male gametocides to induce pollen sterility in okra. Results of study revealed the association of floral bud size and stages of microsporogenesis in female parental lines. Formation of microspore from PMC is between 3 mm to 5 mm bud size. Further, it was reported that Meiosis I completed when but is under 5 mm and after this microgametogenesis start appearing when the bud size between 10 mm to 40 mm buds. However, pollen remains nonviable till bud reaches 45mm size and attains maturity just before flower anthesis between 45mm to 50 mm bud size. Days taken in formation of microspore to viable pollen grain are 18-20 days. Male gametocides used in the study were effective in inducing pollen sterility and vary with different stages of microsporogeneis and bud growth. Among the different treatment combinations, 45 mm bud size treated with malic hydrazide at 450 ppm induced higher pollen sterility under in vitro pollen germination test (93.40 %) and this was followed by the acetocarmine (90.77 %) and ethrel @ 3000 ppm (80.68) at 45 mm bud size.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.