from a root cDNA library using a partial pea ADHI cDNA (1) (1987) J. Mol. Biol. 195,[115][116][117][118][119][120][121][122][123] as a probe. This clone encodes a polypeptide of 380 amino acids.
A karyotype of Trifolium repens constructed from mitotic cells revealed 13 pairs of metacentric and 3 pairs of submetacentric chromosomes including a pair of satellites located at the end of the short arm of chromosome 16. C-bands were identified around the centromeric regions of 8 pairs of chromosomes. A 350 bp tandemly repeated DNA sequence from T. repens labelled with digoxygenin hybridized to the proximal centromeric regions of 12 chromosome pairs. Some correlation between the distribution of the repeat sequence and the distribution of C-banding was demonstrated.
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