This experiment was aimed to investigate the effect of phytase, citric acid and their interaction on growth performance, feed utilization, body composition, economic evaluation and its antibacterial activity against fish pathogens of Nile tilapia fingerlings. Nine isonitrogenous 30 % CP and isocaloric 450.27 Kcal/100g experimental diets were prepared. Phytase was added at the level 0, 1000 and 2000 FTU/kg diet while citric acid was added at the level of 0, 30 and 60 g in the experimental diets. Six hundred and seventy five of Nile tilapia fingerlings were placed randomly in twenty seven V-shaped fiber tanks 120 L capacity of water /tank, in three replicates per treatment was used in this study. Each tank stocked with twenty five fingerlings of with an average initial body weight of 12.47±0.3g /fish. Fish were acclimated to laboratory conditions for 2 weeks prior before the experimental started. Photoperiod was 12h light/ 12h dark regulate. Fish were hand-fed to satiation, two times daily for six days a week through the whole experimental period (60 days). The results indicated that the best growth performance and feed utilization of Nile tilapia fingerlings fed on D5 (1000 FTU phytase / kg + 30g citric acid/ kg) and the lowest group fed on control diet (D1). While, antibacterial activity showed that citric acid alone and citric acid with phytase had highest activity and gave wide inhibition zones against all tested and isolated bacteria which infect Nile tilapia compare with different used antibiotics. Phytase and citric acid positively affected the growth performance, feed utilization,

Root and bark of Limonium axillare (Forssk) Kuntze are used as antidiabetic remedies in parts of East Africa, but this activity has never been fully investigated. To validate its ethnobotanical use, we compared the chemical and pharmacological profiles of the ethanolic extracts of L. axillare root (REE) and aerial parts (AEE). Administration of REE (500 mg kg-1) reduced streptozotocin-induced hyperglycemia by 44%, restored serum insulin levels, reestablished Glut2 and Glut4 expression and ameliorated pancreatic tissue damage in diabetic rats. In vitro studies revealed a strong radical scavenging effect, α-glucosidase, and α-amylase inhibition activity of REE at IC50 at 25.2, 44.8 and 89.1μg/mL, respectively. HPLC analysis identified ten phenolic compounds in REE with umbelliferone as the major constituents at 10 ± 0.081 mg/g of extract. Additionally, six compounds were isolated from REE including, β-sitosterol-3-palmitate, β-sitosterol, myricetin and gallic acids with two new tetrahydrofuran monoterpenes; 2-isopropyl- 3,4,4, trimethyl-tetrahydrofuran (3), and 2-isopropyl-4-methyl-tetrahydrofuran-3,4 dicarboxylic acid (4), the latter was revealed by molecular docking to be a good ligand to glycerol-3-phosphate dehydrogenase a key enzyme in glycolysis.