Staphylococcus aureus is one of the prominent causes of hospital-acquired bacteremia. Despite the availability of anti-staphylococcal antibiotics, hospital acquired S. aureus bacteremia is still a major problem with considerable morbidity and mortality. Therefore, the aim of this study was to isolate, identify and determine the Antibiotics susceptibility profile of Staphylococcus aureus from the surfaces of surgical equipment and environment of major public and private hospitals in Lokoja, Kogi State, Nigeria using colonial characteristics, microscopy and conventional biochemical techniques. The Antibiotics susceptibility profile of the isolates was determined in accordance with the Guidelines of Clinical and Laboratory Standard Institute (CLSI). A total of three hundred and fifty (350) swab samples comprising of fourty (40) from surgical equipment and three hundred and ten (310) from the environment were collected from three (3) different public and private hospitals within Lokoja metropolis. The results obtained showed that 110(31.4%) of samples from the hospital environment were confirmed positive for Staphylococcus aureus with Hospital A constituting 30(8.6%), Hospital B had 59(16.8%) and Hospital C recorded 21 (6.0%). Of the 19 selected S. aureus isolates for antimicrobial susceptibility screening, 10.52% and 5.26% were intermediately resistant to Norfloxacin and Chloramphenicol respectively. Furthermore, the screened S. aureus isolates showed 100% susceptible to Ciprofloxacin, Gentamicin and Erythromycin; 94.73% susceptible to Chloramphenicol and 89.47% susceptible to Levoflaxin. The result also revealed 100% resistance to Penicillin and 15.78% resistance to Rifampicin. The high presence of Staphylococcus aureus in the hospital environment is a potential threat to the health of the patients and the public as this organism has been implicated in several human diseases, especially hospital- acquired bacteremia. Therefore, improved personal and public hygienic practices within the hospitals are required to reduce the high presence of S. aureus and other pathogenic microorganisms.

Antibiotics are secondary metabolites that inhibit other competing cells to give competitive advantage to the microorganisms that produce them. This study is aimed at screening for antibacterial producing bacteria isolated from soil in Keffi. Standard microbiological methods were used in isolation and screening for antimicrobial of activity of crude extracts. Molecular identification of bacteria isolated were carried out using 16srRNA method. The antibacterial activity was carried out using agar well diffusion methods. The highest bacteria were Isolated from maize rhizosphere (90.0%) and the least was from groundnut rhizosphere (50.0%). Bacterial isolated and identified using 16srRNA were Pseudomonas fluorescens, Pseudomonas antarctica, Bacillus megaterium and Acetobacter aceti. The screening for antimicrobial producing bacteria showed that bacterial with laboratory code Gn3 (Pseudomonas fluorescens) showed activity against E. coli (8.57±1.01 mm), S. typhi (5.10±0.11 mm) and S. aureus (6.12±0.32 mm). Ma1 had activity against E. coli (8.57±1.01 mm), S. typhi (5.10±0.11 mm) and S. aureus (6.12±0.32 mm). Ma2 had activity against E. coli (7.56±1.33 mm) and S. typhi (4.54±0.34 mm) and Rc2 had activity only against S. aureus (12.12±0.98 mm) The Antibacterial activity of bacteria isolated and grown in complex medium with nitrogen source revealed that Pseudomonas fluorescens showed activity against the test organism E. coli (14.03±1.06mm), S. typhi (9.11±1.11mm) and S. aureus (5.12±1.02 mm). It was observed in this study that bacterial isolated from soil in Keffi were able to produce substance that that inhibit other bacterial.

Antibiotics were considered to be organic compounds produced by microorganisms which were toxic to other microorganism. This study is aimed at isolation and screening for antimicrobial producing fungi isolated from soil in Keffi. Standard microbiological method was used in isolation and screening for antimicrobial of activity of the crude extracts. Fungi were identified using lactophenol cotton blue stain, and view under x10 and x100 objective microscope lens. highest fungi were isolated from groundnut rhizosphere (58.3 %) and the least was from beans rhizosphere (25.0 %). The screening for antimicrobial producing fungi, isolate fgm8 shows activity against the test organism with 11.02+0.12mm inhibited zone against E. coli (5.33+10mm) against S. aureus and (9.03+0.03 mm) against C. albica. The antimicrobial activity of the fungi grow in complex medium with nitrogen sources showed different ranges of activity against the test organism were Rhizopus sp. only showed inhibition zone of 12.11+1.34 mm against C. albicans. These fungi species should be used to develop starter culture that will be used in local food production, as they show high antimicrobial activity against some pathogenic bacteria that cause infection and food spoilage.