Summary. The PGC's (polymorphism, pseudopodia and filopodia, paucity of microfilaments, high «-glycogen particle content) have been described. In addition, the cell pattern in mesodermal tissues is thought well-suited to PGC invasion owing to a wide extracellular compartment, distributed in the mesenchyme and the early epithelium, and to discontinuities in the basal lamina.Introduction.

The experimental realization of chemerical embryos (Martin's13 technic) permits a quantitative appreciation of the modalities of the colonization of quail gonads by chick germ cells. Results clearly show that nature and origin of the somatic part of the gonad areas settle the characters of the genital ridges populating, and specially the specific index of asymmetry expressed by the percentage of PGC colonizing the right gonad.

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The distribution of glycoconjugates was investigated in the embryonic trunk mesoderm used as a substrate by migrating primordial germ cells (PGCs) by means of ultrastructural cytochemistry. In both mesentery and developing gonads polyanionic sites were abundant in epithelial and mesenchymal cell coats, basal laminae, and extracellular matrices (ECM). In the latter, polyanions distributed on microfibrils and granules were associated with collagen fibers, forming an entangled network. No preferential association of this fibrillo-granular material with PGCs was observed, suggesting that polyanions present in ECM likely act by promoting inflation of the extracellular spaces rather than by providing mechanical guides for the moving cells.

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