The aim of this study was to investigate the Seroprevalence of brucellosis among the slaughtered food animals at Assiut Governorate abattoirs, Egypt. A total of 896 serum samples (747 from cattles & 149 from buffaloes) from different localities in Assiut Governorate abattoirs were tested for detection of the brucella antibodies. Results obtained by buffer acidified plate antigen test (BAPAT) and Rose Bengal plate test (RBT) as screening tests indicated a positive reactors percentage 2.19, 2.24, 2.14 and 4.0 % in Assiut, Abnoub, EL-Qusseia and Dairout respectively. The brucella positive reactors were subjected to confirmation by tube agglutination test (TAT) and Rivanol test (Riv.T). The present study revealed that RBT and BAPAT were the most sensitive as screening tests for diagnosis of brucella in slaughtered animals.

This study aimed to determine Aeromonas spp. in raw milk and some milk products. A total of 100 raw milk, kareish cheese, ice cream and baladi yoghurt (25 samples, each) were collected from different dairy shops and street peddlers in Assiut city, Egypt and were bacteriologically examined for presence and count of Aeromonas spp. The incidences of counted Aeromonas spp. in raw milk, kareish cheese, ice cream and baladi yoghurt were 36, 32, 24 and 0.0%, respectively, with average counts of 1.0×10 5 , 3.2×10 4 , 5.0×10 2 and < 100 cfu/ml, respectively. The incidences of counted Aeromonas hydrophila, in raw milk, kareish cheese and ice cream were 16, 12 and 8%, while for Aeromonas caviae, the incidences were 12, 16 and 12%, respectively. Moreover, the incidences of counted Aeromonas sobria in raw milk, kareish cheese and ice cream were 8, 4 and 4%, respectively. Baladi yoghurt samples were negative for Aeromonas spp. in this study. All the recovered Aeromonas hydrophila organisms were confirmed by PCR assay for the presence of 16S rRNA gene and 100% of the tested strains harboured this gene. The aerA and ahh1 virulence genes were present in Aeromonas hydrophila in percentages of 66.67 and 77.78%, respectively. All the recovered Aeromonas hydrophila, Aeromonas caviae and Aeromonas sobria strains, in this study, exhibited 100% virulence properties on bases of proteolytic, lioplytic, psychrotrophic and β-haemolytic activities. The recovered Aeromonas hydrophila, Aeromonas caviae and Aeromonas sobria exhibited 100% resistance towards Ampicillin, Amoxicillin and Erythromycin antibiotics, while, they exhibited 100% sensitivity towards Ciprofloxacin. The public health hazards of occurrence of Aeromonas spp. in milk and its products as well as the suggestive control measures were discussed.

This study aimed to detect Coxiella burnetii in raw cow's milk by using Polymerase Chain Reaction (PCR). A total of 50 raw milk samples collected from dairy farms were tested. The obtained result showed that, 7 (14%) of the tested samples were positive for Coxiella burnetii. The public heath importance of the disease and control measures was also discussed in this study.