In order to examine the elimination rate of Perfluorodecalin (FDC) emulsions stabilized with different emulsifiers--yolk phospholipid (EYP) and procsanol P-268 (analog of Pluronic F-68)--we performed controlled trials on excretion of FDC in exhaled air, elimination from blood and accumulation in the liver. Adult Wistar rats were injected intravenously with FDC emulsions stabilized with different emulsifiers at a dose of 11.5 g FDC per kg body weight. The concentration of FDC in blood, exhaled air and liver tissue was examined by means of the gas chromatography method. The circulation time in blood for the emulsion stabilized with EYP was much longer comparatively to the emulsion containing the synthetic emulsifier procsanol P-268. The injection of EYP dispersions prolonged the circulation time of lipid-stabilised FDC emulsions. The rate of PFC elimination in exhaled air from such an emulsion and the rate of its accumulation in liver during the initial period after injection are reduced compared to the procsanol-stabilized emulsion. These data are interpreted as the result of a reduction of phagocytic activity of blood monocytes toward the lipid-coated particles of the emulsion. The circulation time of PFC emulsion can be prolonged by using lipid emulsifier and additional injections of EYP dispersion.

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FDC/FTPA (7:3) emulsions stabilized by procsanol (Emulsion 1) and by procsanol with yolk phospholipids (Emulsion 2) were incubated with the donor plasma. After the incubation during 6 hours of Emulsions 1 and 2 with plasma the 36% and 50% decrease of the cholesterol content in plasma was found. Analysis of the lipid content of lipoproteins after the Emulsion 2 administration to rats (2.5 ml/100 g of weight) revealed the 50% decrease of the cholesterol amount in the HDL fraction at 3 and 24 hours posttransfusion. The ratio cholesterol/total phospholipids in the erythrocyte membrane diminished up to 50% as well. The equal degree of the cholesterol adsorption by emulsion from plasma, HDL of rats and erythrocyte membrane is an evidence of nonspecific interaction of PFC particles with the blood components containing cholesterol.

Studies concerning lipid absorption by perfluorochemical particles (PFC) during circulation in the blood stream have been reported in experimental animals. We studied the cholesterol absorption from high density lipoproteins (rats) and erythrocyte membrane [1] and composition of the absorbed lipids [2]. The aim of present investigation is to clarify the influence of the PFC chemical structure and synthetic emulsifier on the composition of the absorbed lipids. The perspectives of clinical application of the effect of the lipid absorption by PFC particles is discussed.

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