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Two pyridine‐degrading microorganisms Arthrobacter crystallopoietes (VKM Ac‐1334D) and Rhodococcus opacus (VKM Ac‐1333D) were isolated from soil. The Gas chromatography‐mass spectroscopy analysis showed that the former species formed 3‐hydroxypyridine, 2,3‐ and 2,6‐dihydroxypyridines during its growth in media containing pyridine, while the latter formed 2‐hydroxy‐ and 2,6‐dihydroxypyridines as degradation intermediates. Products of the pyridine ring cleavage (5‐amino‐2‐oxo‐4‐pentenoic acid and 3‐pentenoic acid monoamide) were also detected.

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