Digestibility of diets based on corn and soybean meal or soybeans treated by roasting or extrusion, with or without an enzyme supplementation, was measured by "true" (Sibbald) methods, by analysis of excreta, and by analysis of ileal digesta. Only analysis of ileal digesta was able to consistently measure differences between soybean and enzyme treatments in the digestibility of CP, starch, fat, and ME. The amino acid (AA) digestibility of the diets was measured by analysis of the ileal contents. Whereas enzyme supplementation improved overall CP digestibility by 2.9%, this improvement was not equal for all AA. Of the AA most important for broilers fed corn-soybean diets, the digestibilities of Lys, Met, and Arg were not improved or not improved significantly by the enzyme supplementation; however, that of Val was improved by 2.3% and that of Thr was improved by 3.0%. A performance trial demonstrated that enzyme supplementation with equal diet formulation improved BW and the feed conversion ratio by 1.9 and 2.2%, respectively. A second performance trial compared standard diet formulations with formulations using enzyme supplementation and energy levels that were reduced by the amount of improvement provided by the inclusion of enzyme in the first performance trial. No difference was seen between treatments, showing that the improvement of nutrient utilization brought about by enzyme supplementation completely compensated for the reduced energy content. Whereas enzyme supplementation should allow a reduction in CP formulation as well, individual AA were not improved equally by supplementation and should also be balanced.

The factorial approach has been used to partition the energy requirements into maintenance, growth, and production. The coefficients determined for these purposes can be used to elaborate energy requirement models. These models consider the body weight, weight gain, egg production, and environmental temperature to determine the energy requirements for poultry. Predicting daily energy requirement models can help to establish better and more profitable feeding programs for poultry. Studies were conducted at UNESP-Jaboticabal to determine metabolizable energy (ME) requirement models for broiler breeders, laying hens, and broilers. These models were evaluated in performance trials and provided good adjustments. Therefore, they could be used to establish nutritional programs. This review aims to outline the results found at UNESP studies and to show the application of models in nutritional programs for broiler breeders, laying hens, and broilers

One of the strategies used to improve fish production is lowering the feed costs and the environmental impact by reducing dietary protein content. Using the deletion method, we determined the optimal amino acid (AA) ratio for Nile tilapia (Oreochromis niloticus) (body weight 20 g). Eleven experimental diets and four replications, taken two at a time, distributed in a randomized block design were used. For this trial, a balanced diet (BD) was formulated. Ten other diets were formulated by the deletion method, in which the BD diet was adjusted to result in a reduction of 45% of the test amino acid. Fish were fed three times a day for 57 days. Groups of fish at the beginning and at the end of the experiment were euthanized for further determination of the carcass nitrogen (N) composition. The optimal ratio of each AA was derived by dividing the requirement for each AA by the requirements for lysine. The essential AA ratios, expressed relative to lysine (=100), were methionine 64, threonine 93, tryptophan 24, arginine 125, histidine 34, isoleucine 57, leucine 96, valine 76 and phenylalanine 101. Our findings might be used to design strategies aimed at reducing the production costs of Oreochromis niloticus.

The objective of this study was to determine models for ME requirements for broiler breeder pullets using the factorial method. The influence of the temperature on maintenance ME requirements was determined by experiments conducted in three environmental rooms with temperature kept constant at 15,22, and 30 degrees C, using the comparative slaughter technique. The energy requirements for weight gain were determined based on the body energy content and efficiency of energy utilization for weight gain. Two ME requirement models for each age were developed using the coefficients for maintenance and weight gain. The models for 3 to 8 wk were ME = W(0.75) (186.52 - 1.94T) + 2.47WG, and ME = W(0.75) (174 - 1.88T) + 2.83WG; for 9 to 14 wk, ME = W(0.75) (186.52 - 1.94T) + 2.69WG, and ME = W(0.75) (174 - 1.88T) + 2.50WG; and 15 to 20 wk, ME = W(0.75) (186.52 - 1.94T) + 2.76WG, and ME = W(0.75) (174 - 1.88T) + 3.24WG. In these equations, W is BW (kg), T is temperature (degrees C), and WG is daily weight gain (g). These models were compared to the breeder's recommendations in a feeding trial from 5 to 20 wk of age. Models 1 and 2 provided energy intakes that promoted BW smaller than the breeder's recommendation. However, all breeder pullets had weights above the standard recommendation. Model 2 gave the smallest ME intake and BW close to the standard recommendation and provided the best prediction of ME requirements.

The present study was carried out to compare the growth curve, performance and carcass yield of two broiler strains, Paraíso Pedrês (PP) and ISA Label (ISA), raised in confined or semi-confined systems. It was used a completely randomized design with five replicates, consisting of 20 and 29 birds in semi-confined and confined systems, respectively. Semi-confined birds had free access to pasture at 28 days of age. Performance data were recorded weekly by building growth curves as well as calculating the growth rate. After reaching the recommended body weight (2.5 kg), male birds were slaughtered for physicochemical analysis of carcass, parts and organs. Semi-confined PP and ISA birds showed higher growth potential, higher weight gain, lower feed intake and better feed-to-gain ratio than confined birds. No interactions were observed for carcass yield and meat quality characteristics. Males presented higher yields of feet, drumstick, thigh, drumstick + thigh, and heart, while females presented higher breast and abdominal fat yield. Breast meat from ISA Label birds was more yellowish than from PP, whereas less soft meat was observed in semi-confined birds