One hundred and nineteen rice breeding lines with two local checks were subjected to the principle component analysis (PCA) and cluster analysis to estimate the existing genetic diversity for yield contributing characters. The first three principal components having Eigen value more than one are cumulatively contributing 68.69% to the total variability. PC1 has the contribution from the traits days to 50% flowering (0.497), days to maturity (0.484) and ear bearing tillers (0.359) which accounted 31.84% of total variability indicating these traits contributed more to the total variance. Cluster analysis revealed that the rice lines were classified into 12 divergent clusters by both PCA and Tocher's method. Among the 12 clusters, cluster 1 had highest number of breeding lines (18) and cluster 11 had least number of lines (2) in PCA cluster analysis whereas in Tocher's method highest number of lines observed in cluster 5 (34) followed by cluster 1(33). This analysis reveals the presence of wide genetic variance in rice breeding lines.

Inhibition of angiotensin converting enzyme(ACE) in presence of captopril(C), lisinopril(L) and enalapril(E) were investigated in testis and epididymis of sheep using Hip‐His‐Leu as substrate. Captopril, lisinopril and enalapril were competitive inhibitors of the enzyme from both tissues. Differences in the I50 and Ki values using these three inhibitors reflects the affinities of these inhibitors for the, ACE. In addition, the relative potencies of captopril, lisinopril and enalapril were different for testicular ACE(C > L > E) and epididymal ACE(L>C >E). This observation suggests differences between the active sites of the testicular and epididymal ACE which may reflect on their functions in vivo.

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Rao and Gowrisankar: Stability-indicating HPLC Method for cardiovascular drugsA simple, accurate, specific and rugged reverse phase liquid chromatographic method was developed for the simultaneous estimation of atenolol, lisinopril, aspirin and simvastatin in bulk and tablet dosage form. A reverse phase gradient program has been developed to separate all the four active ingredients. A gradient programming has been done using 0.05 M Phosphate buffer pH 2.5 adjusted with dilute phosphoric acid, acetonitrile in the ratio 70:30 from 0 min to 10 min, further increase the acetonitrile ratio from 30 to 70 from 10 min to 20 min, on a Hypersil BDS C8 (250×4.6 mm, 5 µ) with a flow rate 1 ml/min, monitored at 232 nm. The mean retention times of atenolol, lisinopril, aspirin and simvastatin were found to be 3.9, 5.8, 9.5 and 18.3 min, respectively. The linearity was established for atenolol 12.5 to 75 µg/ml, lisinopril 2.5 to 15 µg/ml, aspirin 18.75 to 112.5 µg/ml, simvastatin 5 to 30 µg/ml. The proposed method was validated in terms of linearity, range, accuracy, precision, specificity, robustness and ruggedness and the method was successfully applied to the estimation of atenolol, lisinopril, aspirin and simvastatin in combined tablet dosage form.

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