Afzelia africana seeds were obtained from three locations, Abakaliki, Enugu and Nsukka, all in the Eastern part of Nigeria and de-hulled. The seed oil was extracted with (40 -60°C) petroleum ether and the oil was separated from the solvent using a rotary evaporator. Result of analysis shows that the acid value, saponification value, specific gravity, free fatty acid and refractive index of the oil samples were lowest for the samples obtained from Nsukka. The average moisture content of the seeds from the various locations was 5.56 ± ± ± ± 0.5%. The crude protein (26.44%) and oil content (33.32%) did not vary significantly with location or environment. The various oils were found to contain no less than 0.60 mg/100 g of oxalate, 0.70 mg/kg of phytate and neither tannins or cyanogenic glycosides. This paper empirically highlights the possible industrial applications as well as the safety of the seed oil.