Aim: To investigate the antimicrobial activity 2% Chlorhexidine gel (CHX), Aloe vera, Propolis, Septilin and Calcium hydroxide [Ca (OH) 2] on Enterococcus faecalis infected root canal dentine at two different depths (200 µm and 400 µm) and three time intervals (day 1, 3 & 5). Methodology: One hundred and eighty extracted human teeth were infected for 21 days with E.faecalis. Samples were divided into six groups. Group I (Saline) (Negative control), Group II (Propolis), Group III (2% CHX), Group IV (Aloe vera), Group V (Calcium hydroxide), Group VI (Septilin). At the end of 1, 3, and 5 days, the remaining vital bacterial population was assessed. Dentine shavings were collected at two depths (200 µm and 400 µm), and total numbers of colony forming units were determined. The values were analysed statistically with oneway analysis of variance followed by Tukey multiple comparison test. The paired t-test was used to check for differences in growth at different time intervals within groups and for differences at the two depths (P < 0.01). Results: The number of colony-forming units was statistically significant in all groups compared to the control group (Saline). Group III (CHX) and Group VI (septilin) (100%) produced better antimicrobial efficacy followed by aloe vera (78.94%), propolis (66.7%), calcium hydroxide (58.5%). There was significant difference between aloe vera and propolis and no significant difference between data at 200 µm and 400 µm. Conclusion: Septilin and Aloe vera were effective against E.faecalis in dentine of extracted teeth.

In human anatomy mouth is the earliest segment of alimentary canal which receives food and fabricates saliva, and is one of the foundation for bacterial development due to accretion of sugars form food chunks. There have been more than 300 kinds of bacteria set up in the mouth. The purpose of the present study is to investigate the number of microbes’ grown-up at different timings of the day by collecting the samples from individual volunteer oral cavities and counting the colonies; the utmost count was noted as in the range of 190-200 in the evening samples. The same samples were used to calculate minimum inhibitory concentration of Triclosan, the antibiotic formulated for oral hygiene. Triclosan dilutions were prepared and analyzed for the inhibition zones, it resulted that 300mg of the drug have shown satisfactory results. Triclosan was formulated into a dispersible tablet to enable dispersion of tablet into water for rinsing the oral cavity to remove the contaminants. The Dispersion time of the tablet was within the range of 43.66-111.33 second and it was observed that formulation was acceptable with reasonable limits of standard required for dispersible tablets. Addition of superdisintegrant enhanced the dispersion time which facilitates easy mixing of tablet in water. It concludes that superdisintegrant addition technique is a useful method for preparing dispersible tablets by wet granulation method, and Triclosan dispersible tablet further reduces the microbial attack on tooth by rinsing each time after meals, upon doing so on long term basis the dental decays will probably be eradicated.