Modern European integration aspirations of Ukraine cover various aspects of social development, in particular medicine. Research the newest methods of training medical workers for the Ukrainian system of training medical workers is an extremely relevant topic. The purpose of the article is to analyze the Ukrainian experience of the peculiarities of pedagogical training of medical workers in the context of European integration processes. The main methods used in the work are general scientific methods (synthesis, analysis, deduction, and induction). The results determined the essence of the main pedagogical components used in the structure of the professional education of medical workers. The peculiarities of the formation of the pedagogical competence of medical workers in the light of European integration were separately investigated, attention was paid to its main elements, which are important to introduce into the Ukrainian training system and which meet the requirements of the Bologna system. The social and practical aspects of pedagogical training of medical workers, which are already applied as a result of European integration processes in the education of Ukraine, are characterized. The conclusions emphasize the need for further transformations in the field of training medical workers, in connection with the further use of European experience.

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The aim: To study the influence of chemical, physical factors on the biofilm forming activity of P. aeruginosa, A. baumannii. Materials and methods: Biofilm forming activity of P. aeruginosa (10 isolates) and A. baumannii (10 isolates) was studied in nutrient media of different composition. There was used the method in 96-well crystalline violet staining plates with spectrophotometry (STAT FAX®4300, wavelength of 620 nm). Results: Results showed that in standard medium (trypto-soy broth), strains of P. aeruginosa (90%) and A. baumannii (60%) obtained high biofilm forming activity. A. baumannii formed biofilms even in sterile water. Biofilm forming activity of urease positive P. aeruginosa increased in the medium with 1.0% urea. Both Acinetbacteria and Pseudomonas intensively produced their biofilms in the presence of 5% serum or sub-bacteriostatic concentrations of levofloxacin in the media. High concentrations of sodium chloride inhibited their biofilm activity. Conclusions: Isolates of Acinetobacter and Pseudomonas obtain the protective biofilm-forming ability under such adverse environmental conditions as insufficient nutrients, high osmotic pressure, the presence of antibiotics but at high concentrations sodium chloride biofilm-formation is stimulated only in the first bacteria and suppressed in the second one.

У статті наведено результати експериментального дослідження взаємного потенціювання протимікробної активності антисептика декаметоксин і антибіотиків при поєднаному застосуванні. Встановлено посилення протимікробної ефективності антибіотиків цефалоспоринової, карбапенемової, аміноглікозидної, фторхінолонової, поліміксинової груп у присутності декаметоксину щодо неферментуючих грамнегативних бактерій та інших мікроорганізмів. Показано можливість досягнення бактерицидного ефекту щодо плівкових форм бактерій при поєднаному впливі антибіотиків і антисептиків.

Purpose of the study. Justification of the effective treatment tactics of the wounded with the gunshot fractures of the long bones.Materials and methods. The examination and treatment of 123 wounded with gunshot fractures were performed. All wounded were divided into 3 groups according to clinical observation. For the first clinical group the traditional treatment of the combat fractures with osteometallosynthesis out of injury zone was done. In the second group, treatment was added with negative pressure therapy. In the third clinical group Wounded got a VAC-associated therapy with the proposed of counter-drainage of wounds with the flow-washing irrigation with antiseptic solutions Decasan + 3% hydrogen peroxide. A bacteriological study of the wounds` exudates was carried out, and the sensitivity of microorganisms to antibiotics was determined.Results. The assay of microbiological investigation of the wounds of patients, which got explosive and mine-explosive injuries, demonstrated a predominance gram-negative microflora in the wound microbiocenoses such as Acinetobacter spp. (53% of cases) and Pseudomonas spp. (15% of cases). Gram-positive cocci were isolated from 22,2% of cases. The analysis of the antibiotic sensitivity data of gram-negative non-fermentative rods showed a high level of resistance to most antibacterial. All strains of acinetobacteria and pseudomonads were susceptible to polymyxin B and colistin, but resistant to unprotected and protected aminopenicillins (amoxicillin/clavulanate, ampicillin/sulbactam). Acceleration of regenerative processes in the wound under the influence of VAC-therapy (formation of healthy granulations, disappearance of edema) in patients with negative pressure suppression (II HS, III HS) led to a reduction of hospitalization period, which took in average 7,8 ± 1,2 days, that was 5,2 ± 0,8 days less than in a control group. In the third group of wounded, a mixture of Decasan and 3% hydrogen peroxide in the proportion of 3 : 1 was used for rinsing of wounds. On the third day tissue edema decreased in 94,45% of the wounded in that observation group, while in the second clinical group it was observed in 88,89% of patients. The duration of the hydration phase in the wound process was reduced to 5,7 days. The period of complete healing of the wounds was shorter for 2,5 days. The period of indoor stay of the wounded of this group in the hospital decreased from 14,97 to 10,8 days.Conclusions. Prevalence of gram-negative microorganisms in a gunshot wound and their high degree of resistance to antibiotics should be noticed when one takes a decision about empirical antibiotic therapy in the wounded. Observed clinical results of the proposed negative pressure therapy with counter-drainage of wounds by setting of flow-washing irrigation with a mixture of antiseptics Decasan and 3% hydrogen peroxide allow recommending this scheme for treatment of wounded with gunshot fractures of long bones.Keywords: Gunshot wound, antiseptics VAC-therapy.