Nanocrystalline titanium dioxide (TiO 2 ) photoanodes with four different film thicknesses from 5.57µm to 20.65µm were prepared by doctor-blade technique. Performance of dye sensitized solar cells (DSSCs) fabricated with these photoanodes were studied using current-voltage characteristics and incident photon-to-current conversion efficiency (IPCE) measurements. Electrochemical impedance spectroscopy (EIS) was used to analyze the effect of TiO 2 film thickness on the charge transfer resistance and electron life time in the solar cells. Voltage decay measurements were used to study the recombination process of photo generated charge carriers. These studies revealed that the photovoltaic properties of DSSCs largely depend on the film thickness of TiO 2 photoanode. Further, the DSSCs fabricated using a TiO 2 film of 12.73 µm thickness exhibited the best photovoltaic performance with highest incident photon-to-current conversion efficiency, highest short-circuit photocurrent, lowest charge transfer resistance, highest electron life time and lowest recombination life time.