Chequerboard and time-kill methods were used to compare the in vitro efficacies of the combinations gatifloxacin (GAT) with cefoperazone (CFP) and GAT with cefoperazonesulbactam (CFP-SUL) against 58 clinical isolates of Pseudomonas aeruginosa. The combinations GAT+CFP and GAT+CFP-SUL were shown to be synergistic for 36.2 and 58.6 % of isolates tested, respectively, using the chequerboard method. Time-kill studies with 11 strains showed synergy in 54.5 % for the GAT+CFP combination and 72.7 % for the GAT+CFP-SUL combination. The agreement between these two methods was found to be 72-81 %. There was a significant difference in synergy between the two combinations tested (P50.011).
A simple, rapid and accurate RP-HPLC method was developed for the determination of racecadotril in solid pharmaceutical dosage form. The method showed a linear response for concentrations in the range 10 -200 g/ml using methanol: water (80:20 v/v) as the mobile phase with detection at 231 nm and a flow rate of 1.2 ml/min. Solution concentrations were measured at a weight basis to avoid the use of an internal standard. The method was statistically validated for accuracy, precision, linearity, range, ruggedness, robustness, forced degradation, solution stability, and selectivity. Quantitative and recovery studies of the dosage form were also carried out and analyzed; the % RSD from recovery studies was found to be 0.53. Due to the simplicity, rapidity and accuracy of the method, we believe that the method will be useful for routine quality control analysis.
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