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A simple, rapid, and sensitive fluorimetric method was developed and validated to quantify quassia in polymeric nanocapsule suspensions and non polymeric suspensions, using acetonitrile as a solvent. The excitation and emission wavelengths were set at 397 nm and 465 nm, respectively. The calibration graph was linear from 0.05 to 0.25 µg/mL with a correlation coefficient of 0.9892. The detection and quantitation limits were 2.4 and 0.8 µg/mL, respectively. The validation results confirmed that the developed method is specific, linear, accurate, and precise for its intended use. The current method was successfully applied to the evaluation of curcumin content in lipid and polymeric nanocapsule suspensions during the early stage of formulation development.

Sweet corn fibres of about 15 gm were extracted with methanol for 5 hours in heating mantle at 40C and filtered and allowed to dry. The dried gel was further analyzed for estimation of Retinyl palmitate by spectrophotometrically by laboratory method and found to be 140 mg/kg. The dried extract gel was further estimated for ascorbic acid both by UV and HPTLC and found to be linear in the range of 1-5 ug/ml and 5-10ug/ml, correlation coefficient was found to be 0.997 and 0.998 and the amount of ascorbic acid was found to be 338 ng/ml and 9.9 ng/ml by UV and HPTLC respectively. The method was found to be linear.

Lip balm was prepared and its antioxidant activity was determined by PFRAP, CUPRAC, Flourimetry methods and results of emission spectra was examined and fournd to possess almost same concentration of 0.3 ug/ml. And the ascorbic acid was determined by HPTLC method and the Rvalue of the lip balm was found to be 0.98 and is near to the abscorbic acid sample. And hence proved that beetroot lip balm was found to possess antioxidant activityThe anti oxidant activity was determined and was found to be simple, novel and economical.

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