Herbal plants were used for food flavouring and food preservation and for the treatment of some illnesses. Ocimum tenuiflorum was one of the ancient herbal plants whereby its medicinal properties have been widely studied such as wound healing. Wound healing involves a sequence of recovery stages and many factors affect the wound healing process. The literature review showed that, leaves part was widely studied by researchers for its wound healing potential. The aim of this study was to screen the wound healing potential of other parts of the plants which are the stems and flowers in comparison to leaves using in vitro wound healing assays. Aqueous extraction for all the parts was done using the maceration method. The stems and flowers extracts did not show any promising wound healing effects through cell migration and angiogenesis process but showed significantly (p<0.05) higher effect via cell proliferation activity. Conversely, leaves extract have shown positive wound healing potency by significantly enhanced (p<0.05) the cell migration and angiogenesis activity. Overall, this experiment proved that the leaves own wound healing potency via cell migration and angiogenesis activity which could be beneficial in wound healing process. Thus, O. tenuiflorum leaves extract can be a favorable wound healing treatment yet, further detailed studies are required.