BackgroundBrucellosis is a bacterial zoonotic disease. Annually in the world more than 500,000 new cases of brucellosis in humans are registered. In this study, we propose an evolutionary model of the historical distribution of B. melitensis based on the full-genomic SNP analysis of 98 strains.ResultsWe performed an analysis of the SNP of the complete genomes of 98 B. melitensis strains isolated in different geographical regions of the world to obtain relevant information on the population structure, genetic diversity and the evolution history of the species. Using genomic sequences of 21 strains of B. melitensis isolated in Russia and WGS data from the NCBI database, it was possible to identify five main genotypes and 13 species genotypes for analysis. Data analysis based on the Bayesian Phylogenetics and Phylogeography method allowed to determine the regions of geographical origin and the expected pathways of distribution of the main lines (genotypes and subgenotypes) of the pathogen.ConclusionsWithin the framework of our study, the model of global evolution and phylogeography of B. melitensis strains isolated in various regions of the planet was proposed for the first time. The sets of unique specific SNPs described in our study, for all identified genotypes and subgenotypes, can be used to develop new bacterial typing and identification systems for B. melitensis.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-4762-2) contains supplementary material, which is available to authorized users.
Aim. Determination of the degree of phylogenetic relationship of Yersinia pestis strains isolated from the territories of natural foci of plague from the Caucasus using VNTR-typing by 25 loci (MLVA25). Materials and methods. 26 strains of Y. pestis from Russian natural foci of the Caucasus were used in the study. 25 loci of tandem repeats in Y. pestis genome by Le Fleche scheme were used for execution of multi-locus VNTR-analysis. Deciphering of nucleotide sequences was carried out in automatic sequencer ABI 3130 Genetic Analyser. Analysis of confinement of clusters to certain territories, objects and time of isolation of strains was carried out using Arc GIS 10.1 program. Results. Groups of MLVA25-types of various levels of discrimination were formed: clusters, groups and subgroups. Clusters were formed by strains of various taxonomic membership: main and subspecies of Y. pestis. Subgroups reflect membership of strains in certain foci, and MLVA25-types - the degree of genetic relationship. Conclusion. Genetic «portraits» of plague causative agents obtained using MLVA25-types circulating in various natural-focal territories allow to solve problems ofboth theoretical and practical character: from interpretation of microevolution processes to the search of the source of infection and ways of its spread during possible epidemic complications.
The article presents the results of experimental evaluation of the effectiveness of new culture substrate
developed for the isolation and cultivation of Legionella, the strains isolated from environmental objects in
the resort city of Sochi before and during XXII Olympic games. Dense nutrient substrate prepared on the
basis of enzymatic hydrolysate of chicken egg yolk and enzymatic hydrolysate of the lung of the pig with
additional stimulants allowed saving number of colonies in the earlier stages of the study and storage of the
isolated strains during long period of time compared with domestic commercial nutrient substrate.
с.е.Гостищева, л.с.катунина, а.а.курилова, н.В.абзаева, Ю.с.ковтун, н.В.Жаринова, о.а.коняева, е.б.Жилченко, а.н.куличенко применение плотной питательной СредЫ на оСнове гидролиЗата кукуруЗного ЭкСтракта Сгущенного в проиЗводСтве вакцинЫ чумной живой и для хранения штаммов чумного микроБа ФКУЗ «Ставропольский научно-исследовательский противочумный институт», Ставрополь, Российская Федерация цель исследования-разработка плотной питательной среды на основе ферментативного гидролизата кукурузного экстракта для использования в производстве вакцины чумной живой и хранения на ней штаммов возбудителя чумы. материалы и методы. вакцинный и вирулентные штаммы Yersinia pestis, питательные среды для накопления и хранения. исследуемые параметры изучались согласно нормативной документации. результаты и выводы. разработана питательная среда на основе ферментативного гидролизата кукурузного экстракта сгущенного с ростостимулирующими добавками-солью мора и натрием сернистокислым. изучены ее физикохимические и биологические свойства. апробация среды в производственной лаборатории показала ее высокую продуктивность и возможность применения в промышленном выпуске вакцины чумной живой-получены серии препарата с оптической концентрацией 100 млрд/мл и жизнеспособностью (68,2±0,9) %. применение данной среды позволяет повысить выход биомассы и снизить себестоимость конечной продукции. подтверждена возможность хранения на разработанной среде вирулентных штаммов возбудителя чумы при температуре (4±2) °с в течение 18 месяцев без снижения жизнеспособности культуры. Ключевые слова: питательные среды, чумной микроб, гидролизат кукурузного экстракта сгущенного.
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