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The goal of this work was investigation the effect of adsorption the Fe 3 O 4 as magnetic nanoparticle on lysozyme activity and thermal stability. The turbidimetric assay (activity) of lysozyme was followed by the decreased optical density of a turbid cell suspension (about 0.3 mg/cm 3 Micrococcus lysodeikticus) photometrically at 450 nm. In this study, the effect of nano-Fe 3 O 4 on lysozyme activity was investigated by UV-Vis spectrophotometry at pH = 7.25 at 35°C using sodium phosphate buffer. Measurements were carried out using 6 9 10 -8 M (0.1 mg/cm 3 ) concentration of lysozyme and a range of nano-Fe 3 O 4 concentrations between 0 and 0.06 mg/cm 3 . It was found that by increasing nanoFe 3 O 4 , the rate of M. lysodeikticus lyses (lysozyme activity) increases. The thermal stability of lysozyme was investigated in the presence of nano-Fe 3 O 4 over the temperature range of 293-363 K in sodium phosphate buffer and pH = 7.25. Results indicated that by increasing nano-Fe 3 O 4 , the thermal stability of lysozyme increases. Graphical abstract