In this work, a red emission AIE active mitochondrial probe is developed. It is the first non-self-quenching mitochondria specific probe with membrane potential sensitivity. Its application in sensing the membrane potential differences in mouse sperm cells is demonstrated.

Abstract:This study conducts an exploratory evaluation of the performance of the newly available Sentinel-2A Multispectral Instrument (MSI) imagery for mapping water bodies using the image sharpening approach. Sentinel-2 MSI provides spectral bands with different resolutions, including RGB and Near-Infra-Red (NIR) bands in 10 m and Short-Wavelength InfraRed (SWIR) bands in 20 m, which are closely related to surface water information. It is necessary to define a pan-like band for the Sentinel-2 image sharpening process because of the replacement of the panchromatic band by four high-resolution multi-spectral bands (10 m). This study, which aimed at urban surface water extraction, utilised the Normalised Difference Water Index (NDWI) at 10 m resolution as a high-resolution image to sharpen the 20 m SWIR bands. Then, object-level Modified NDWI (MNDWI) mapping and minimum valley bottom adjustment threshold were applied to extract water maps. The proposed method was compared with the conventional most related band-(between the visible spectrum/NIR and SWIR bands) based and principal component analysis first component-based sharpening. Results show that the proposed NDWI-based MNDWI image exhibits higher separability and is more effective for both classification-level and boundary-level final water maps than traditional approaches.