Cryopreservation is the most effective method for the long-term storage of semen. Furthermore, cryopreservation enhances genetic utilization by facilitating the distribution of desirable genes and control the transmission of certain diseases (Grossfeld et al., 2008). However, the cryopreservation process exposes semen to physical and chemical stress that impairs sperm quality (Hezavehei et al., 2018). Each step of the cryopreservation including dilution, cooling, freezing, and thawing, diminishes the fertilization potential, with approximately 40% to 50% of spermatozoa not even surviving the process (Rath et al., 2009;Watson, 2000). In comparison with fresh semen, insemination with frozen-thawed semen results in lower fertility and farrowing rates by 20% to 30% (Knox, 2015;Yeste et al., 2017). Boar spermatozoa in particular are very susceptible to peroxidative damage because of their high polyunsaturated fatty acid content and low concentrations of cholesterol in their plasma membrane (Mandal