Metastatic cancers adopt certain properties of normal cells in developing or regenerating organs, such as the ability to proliferate and alter tissue organization. We find here that activity of the Hedgehog (Hh) signalling pathway, which has essential roles in developmental patterning, is required for regeneration of prostate epithelium, and that continuous pathway activation transforms prostate progenitor cells and renders them tumorigenic. Elevated pathway activity furthermore distinguishes metastatic from localized prostate cancer, and pathway manipulation can modulate invasiveness and metastasis. Pathway activity is triggered in response to endogenous expression of Hh ligands, and is dependent upon the expression of Smoothened, an essential Hh response component that is not expressed in benign prostate epithelial cells. Monitoring and manipulating Hh pathway activity may thus offer significant improvements in diagnosis and treatment of prostate cancers with metastatic potential.

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Studies were conducted at three locations in Florida to evaluate the effects of kasugamycin alone, in alternation, or as a tank-mix partner with copper bactericides and other fungicides against bacterial spot of tomato. In greenhouse trials, kasugamycin, formulated as Kasumin® 2L, reduced bacterial spot severity by up to 37.5% compared with a non-treated control. Little advantage in disease control was observed by mixing kasugamycin with other fungicides. Kasugamycin was assessed in six field trials. In the four field trials that tested kasugamycin alone, it was as effective as the standard copper + mancozeb treatment for the control of bacterial spot. In four trials, no benefit was observed in applying kasugamycin as a mixture with copper + mancozeb, and only one of three trials did alternating kasugamycin with copper + mancozeb improve bacterial spot control over either the copper + mancozeb standard or kasugamycin alone. Although kasugamycin was effective for the control of bacterial spot in greenhouse and field trials, rapid development of resistance in field populations of X. perforans may shorten the effective use of this antibiotic.

In the present study the upper reference limits (URLs) for resting plasma norepinephrine, epinephrine, serum aldosterone, plasma renin activity, aldosterone/ renin activity ratio, as well as urinary vanillylmandelic acid in healthy Egyptian normotensive subjects over a range of ages (5-60 yr) were established. There was a significant age effect on plasma norepinephrine, UVMA, serum aldosterone and PRA, whereas a single URL for plasma epinephrine level is satisfactory. In uncomplicated untreated essential hypertensive subjects (5-60 yr), the average prevalence of elevation in the plasma norepinephrine, epinephrine and urinary vanillylmandelic acid above their corresponding URLs was 85.10,62.15 and 83.20 %, respectively. This suggests that elevation in plasma catecholamine concentrations is more likely a common consequence than playing a possible role in the pathogenesis of hypertension, supported by insignificant correlation coefficients between the plasma catecholamine levels and resting systolic and diastolic blood pressure values (SBP&DBP) in all hypertensive age groups. Primary hyperaldosteronism was not detected among the normokalemic essential hypertensives at any age using aldosterone /plasma renin activity ratio as a primary screening method. In the present study, 7 statistically significant positive coefficient correlations are reported for SBP or DBP values with UVMA levels in hypertensive children and adolescents, serum aldosterone in old hypertensives, and PRA in adult hypertensives.