Aims:To evaluate the effect of three coating materials: Acrylic resin coating "monopoly", olive oil and grape seeds oil on the Candida albicans growth, and on color of a heat -cured acrylic resin denture base. Materials and methods: Three coating materials underwent pores method to find out their antifungal effect. The effect of coating materials on the acrylic denture base surface was determined via Sensitivity test in which twenty discs of acrylic resin 6mm diameter and 2mm thickness were prepared and divided into four groups: control (uncoated), monopoly, olive, and grape oil coated groups. These discs were added to Sabouraud Dextrose agar surface and incubated for 24 hours at 37 o C and the diameter of growth inhibition zones were measured. Then the Turbidity test was taken place, in which 60 acrylic specimens (1cm x 1cm x 2mm) were prepared and immersed in 60 test tubes that were divided into four groups, each group (15 specimens) was subdivided into three groups of incubation: 7, 20, and 30 days incubation period, each test tube contained 4 ml Brain Heart Infusion Broth, 0.1 ml of 24 hours Candida albicans, and its corresponding acrylic specimen, turbidity (yeast growth) was measured by a spectrophotometer at 530 nm wave length. For color change measurement test, 20 acrylic resin specimens (45 x 10 x 2.5 mm) were prepared and distributed into four studied groups. The absorbed light was measured by spectrophotometer and termed optical density at 345 nm. Results: All coating materials were fungicidal in pores method. Statistical analysis of sensitivity test showed that Candida albicans was susceptible at highest extent to monopoly coated denture base followed by the oils coated groups. In turbidity test, monopoly specimens showed antifungal activity in all periods of incubation, but the effect decreased gradually, olive and grape oil coated specimens exhibited their highest antifungal activity after 30 days incubation. Color change measurements showed that monopoly coated group had the highest optical density, while the olive and grape groups showed a decrease in the optical density when compared to uncoated group. Conclusions: Use of coating materials on the acrylic denture base surface was beneficial, especially monopoly in terms of antifungal activity against Candida albicans followed by natural oils (olive and grape oils). However, coating materials showed a significant change in the color of acrylic denture base.
Aims: To evaluate the effect of three different cleanser on the color stability of three different types of artificial teeth. Materials and methods: sixty samples of artificial anterior teeth were prepared which are Acry-Rock, RMH teeth and Porcelain teeth and immersed in three types of denture cleanser(Bony plus, Protefix and soda+ vinegar) for 8 hours and the color of the teeth was measured by Easyshade's spectrophotometer at 3 intervals(1,2and4weeks). Results: Showed that the a'b values of ceramic teeth and L value of RHM teeth were significantly differencent after 2 week of immersion in three type of denture cleanser. Analysis of variance demonstrated that L'a'b value for ceramic teeth and RHN teeth were statistically significant after 4 week immersion in three type of denture cleanser, The result of color change (∆E) for three denture cleansers of three periods of immersion were clinically accepted. Conclusion: Long period of immersion of artificial teeth in denture cleanser cause significant color change for L*a*b* values but they were clinically accepted.
Aims:The study aims to evaluate the influence of various denture cleansers on colonization of Candida albicans to Cobalt-Chromium alloy denture base and the subsequent roughness assosiated with these cleansers. Materials and methods: Thirty-six samples of Co-Cr denture base and five cleansers, four prepared(alum, salt, soda+vinegar, soda+thymol), one commercial ( protefix ) and distilled water(D.W) as a control were used. Samples were immersed in denture cleansers for one month in which each cleanser had 6 samples excluding 6 samples in D.W as a control. Half of samples for each cleanser were immersed ½ hr per day and the other half immersed 8hrs per day through one month, before microbiological examination, samples were tested for surface roughness using profilometer. Candida albicans cell suspension was incubated with the test samples for 1hr at 37°C after which the test samples were immersed in their cleansers for 1hr. Visualization, inspection and enumeration of adherent C.albicans cells and detection of the anti-adherent effect of the cleansers was achieved by using light microscopy. Results: The results demonstrated insignificant difference in surface roughness of Co-Cr alloy denture base in the cleansers at 1/2 hr and a significant difference at 8 hrs immersion. There was a significant difference in C.albicans colonization to Co-Cr denture base in which all the cleansers showed less adhesion than control. The results also revealed that (soda+Thymol oil) cleanser expressed the least values of colonization and roughness among other cleansers. Conclusions: The cleansers were effective as anti-adherent yeast cells to Co-Cr denture base and showed roughness degrees less than control.
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