The efficiency of copper oxide nanoparticles synthesized from aqueous extract of cordia myxa L. on some breast cancer lines AMJ-13,MCF-7, and HBL-100 as normal cell lines was studied. Different concentration of copper oxide nanoparticles (25,50,75,100) µg/ml at different times (24,48 and72) h were selected. The results showed that the effect of nanoparticles depend on the concentration. As the concentration of nanoparticles increase the percent of inhibition increase. It was found that concentration of copper oxide nanoparticles at 100 μg / ml gave the highest inhibitory rate of cell growth MCF-7 (71.1%) after 24 hours, while the percent of inhibition for AMJ-13 was (69.6)%. When the exposure time was increased to48 h, the rate of inhibition at 100 mg/ml was 80% for MCF-7 while 73% for AMJ13. By increasing the duration of the exposure to 72 hours, the rate of inhibition at l00µg/ml were (85.2 and 78.2)for MCF-7 and AMJ13 respectively. No significant inhibition was found for HBL-100 at different concentration and different times. These results was indicated that copper oxide nanoparticles synthesized from the aqueous extract of cordia myxa L. had a toxic effect on growth of some breast cancer cell lines.

Abstract:Electrochemical studies by cyclic voltammetric (CV) method provide high potential for investigation of oxidant pollutant compounds such as Pb(II). Glassy carbon electrode (GCE) can be used for the potentiostatic analysis of the lead ions in different electrolyte especially human blood medium to studying the effect of the lead ions on the blood components. The results of this study were found that oxidation and reduction current peaks of lead ions in 1M KCl is high value and enhanced by adding ascorbic acid (AA) solution, while in human blood medium was disappearing the reduction current peak and enhance the oxidation current peak by lead ions. It means that lead ions in blood medium act as an oxidative reagent and causes problem in the decomposition of the blood component. Also, it was found that using folic acid (FA) solution with lead ions in blood medium causes the enhancement of reduction current peaks of lead ion and decreasing for oxidation current peaks, so this means that folic acid solution acts as antioxidative reagent in present of lead ions in blood medium, it may recommended to taking tablet of FA for the patients who suffers to lead pollution.