Introduction: COVID-19 is a disease caused by SARS-CoV-2 (Severe Acute Respiratory Coronavirus 2), an outbreak in the world in 2019 until now. High cholesterol levels correlate with increasing disease severity in SARS-CoV-2 infection because there is a surge of cases in Gianyar Regency, a district of Taro Village. This study aimed to examine the relationship between age and gender to cholesterol levels which are expected to become information for the people of Taro Village as a basis for health screening to prevent comorbid diseases that increase mortality in SARS-CoV-2 infection and increase awareness of the people of Taro Village to maintain health and implement a healthy lifestyle. Method: Capillary blood was taken and then checked for cholesterol levels using a standardized tool on 44 respondents. The cholesterol check was conducted in Gianyar Regency, precisely in Taro Village, Tegalalang District, and Bali. The data obtained will be analyzed using univariate analysis followed by bivariate analysis using the Spearman correlation test for variables of age and cholesterol levels. In contrast, for variables gender and cholesterol levels, the Mann-Whitney test is performed. Results: A total of 44 individual respondents in this study, the distribution of data, namely the age of the repondent is more in the 41-50 year age group, the number of female respondents is 33 or 75.0% of the total respondent, and 70.5% of the respondent or 31 have normal cholesterol levels. The results of the Spearman test on the variables of age and cholesterol levels obtained a P value>0, 05, and the results of the Mann Withney test on the variables gender and cholesterol levels obtained a P value>0, 05. Conclusion: Most taro villagers have normal cholesterol levels obtained as many as 31 people, or 70.5% of the total respondent. It may be influenced by the work factor of taro villagers who work a lot in the agriculture and plantation sectors. Based on the study results, there is also no significant relationship between age and gender with the cholesterol levels of Taro Villagers. However, high cholesterol levels were only found in females as many as five people.

Inflammatory bowel disease (IBD) is classified into ulcerative colitis and Crohn's disease. It is chronic inflammation of gastrointestinal tract and idiopathic disease. Sugar is proved to be risk factor for IBD. Calorie restriction is a nutrition intervention to reduce energy intake and can reduce chronic desease. This study aims to determine the difference in the effect of a high-calorie diet and calorie restriction on intestinal inflammation. Eight-week-old healthy female Balb/c mice were divided into two groups. The high calorie (HC) group was fed standard diet plus oral gavage of glucose solution every day for 4 weeks. The dose of glucose solution was 0.0325 ml/gBW or 3-5% additional calories each day. The calorie restriction (CR) group had their calorie intake restricted to 50% of standard diet 3 times a week. After 4 weeks of treatment, the mice had their colon removed. The assessment of the intestinal inflammation was carried out using a light microscope, with a scoring system by Erben et al (2014) in HE staining. The mean score of the HC group was 1.22, this was higher than the CR group with a mean score of 1.18 even tough the result revealed that the scores of intestinal inflammation were not significantly different in the two groups (P = 0.827). However, there is no evidence for the underlying mechanism of intestinal inflammation by high-calorie diet and improvement of intestinal inflammation in high-calorie diet via calorie restriction. Thus, further research is needed on other calorie restriction methods in high-calorie diet study. Keywords: High calorie diet, Calorie restriction, Intestinal inflammation

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