Background: Magnetic nanoparticles show great promise for use as tools in a wide variety of biomedical applications. The purpose of this study was to investigate the potential effects of methacrylamido-folic acid (Ma-Fol)-modified magnetic nanoparticles on 5RP7 (H- ras -transformed rat embryonic fibroblasts) and NIH/3T3 (normal mouse embryonic fibroblasts). Methods: The cytotoxicity and viability of 5RP7 and NIH/3T3 cells were detected. The percentage of cells undergoing apoptosis was analyzed by flow cytometry using Annexin V-fluorescein isothiocyanate staining. Nanoparticle internalization into 5RP7 and NIH/3T3 cells was visualized by transmission electron microscopy. Conclusion: In this study, folic acid coupled to the surface of iron oxide for selective binding to cancer cells and immobilized the surfaces of magnetic nanoparticles. This complex improves cell internalization and targeting of cancer cells. We detected increased apoptosis using flow cytometry and transmission electron microscopy. Results: Folic acid modification of magnetic nanoparticles could be used to facilitate uptake to specific cancer cells for cancer therapy and diagnosis. Our results showed that the uptake of folic-acid modified nanoparticles by 5RP7 cancer cells was also much higher than that of 3T3 cells. This modification can be used for successful targeting of cancer cells expressing the folate receptor.

In this present study, it was aimed to determine the composition of the essential oil obtained by water distillation from the aerial parts of Origanum acutidens. The oil was analysed by GC-FID and GC/MS. Carvacrol (69.3%), p-cymene (8.3%), y-terpinene (6.3%), β-caryophyllene (2.7%) and borneol (2.2%) were found as main constituents. In addition, headspace-solid phase microextraction using a Polydimethylsiloxane-Divinylbenzene (PDMS/DVB -65 μm) fiber of O. acutidens was performed and analysed by GC-FID and GC/MS. The main components were identified as: carvacrol (44.0%), p-cymene (25%), ϒ-terpinene (8%), β-caryophyllene (6.1%) and borneol (6.1%), respectively.

Background and Aims:The aim of the present study was to determine the morphological, micromorphological, anatomical and palynological characteristics of Glaucosciadium cordifolium (Boiss.) B.L. Burtt & P.H. Davis Methods: Measurements were performed on approximately 30 different samples for morphological study. The fruit micromorphologies and pollen properties were examined using scanning electron microscopy. For anatomical studies, all sections prepared from stem, leaf and fruit were taken by hand. Results: Cross-sections of stem, leaf and fruit are examined and a detailed anatomical description is presented and supported by photographs. It is a glaucous perennial herb with a height of 34-180 cm which has an acrid smell when bruised. The fruit is a dry schizocarp, glabrous and oblong-elliptic. The mericarp is elliptic in the transverse section which is winged in the lateral. The fruit surface ornamentation of the genus is occasionally wavy and striped and the upper surface simple, short and scaly. The stomata is observed. Conclusion:In this study, morphological, anatomical and palynological properties of the Glaucosciadium cordifolium are described and illustrated for the first time. In addition, the palynological results confirmed the stenopalynous characteristic of the family Apiaceae, and revealed that the pollen grains of the genus are perprolate in shape.

The study aimed to determine the total phenol content of the extracts prepared with different solvents (70%methanol, ethyl acetate, 5% infusion) from the aerial parts of the I. oculus-christi, as well as to evaluate the antioxidant capacity and antimicrobial activity of the plant and to determine the main components responsible for the effect. The total phenolic content of the extracts prepared from the aerial parts of I. oculus-christi was calculated in gallic acid equivalents and the total phenolic content of the species was found to be in the range of 23.8-75.3 mg GAE/g. The highest total phenol content calculated as gallic acid equivalent was detected in the 5% infusion sample. The antioxidant activity of I. oculus-christi was also tested using DPPHˈ free radical scavenging in vitro assay (IC50: 0.092-1.03 mg/mL). The 5% infusion of the plant was shown to be the most effective extract in the antioxidant activity test performed using the DPPHˈ technique. The findings revealed that there is a correlation between total phenol content and antioxidant activity, as numerous research as suggested. By using the microdilution method, extracts from the aerial parts of I. oculus-christi were tested against Candida strains, and the MIC value for C. utilis was found to be 0.03 mg/mL in 5% infusion and ethyl acetate extracts. In terms of evaluating the biological effects obtained, the chemical composition of extracts was analyzed by HPLC-MS/MS, and the main components (5-caffeoylquinic acid, 1,5-dicaffeoylquinic acid, luteolin/kaempferol, apigenin, and rutin) were determined.