No abstract

The aim of the study was to evaluate the potential protective effect of ozonized olive oil (OZO) in 2,4-dinitrobenzene sulphuric acid (DNBS) induced colitis in rats and to elucidate the role of some antioxidant defense system (superoxide dismutase “SOD,” glutathione peroxidase “GSH-Px,” and catalase “CAT”) in these effects. The physicochemical parameters including viscosity, peroxide, and acid values of olive oil and OZO were evaluated. The animals were divided into several groups and the colitis was induced in the rats by intracolonic instillation of DNBS at dose of 15 mg/rat. Olive oil (OO) at dose of 6 mg/kg and OZO at doses of 3 and 6 mg/kg was administered orally for 7 days, starting the day before induction of colitis. Our results showed that macroscopic and microscopic damage scores were significantly reduced in a dose response manner in rats pretreated with OZO only. In contrast, CAT, GSH-Px, and SOD activities were significantly increased in the distal colon of inflamed animals pretreated with OZO with respect to control group dose dependently. Results demonstrate that OZO pretreatment exerts protective effects in DNBS induced colitis in rats and provide evidence that the protective effects of OZO are mediated by stimulation of some antioxidant enzymes.

No abstract

No abstract

hree antagonists, i.e. Pseudomonas fluorescens, Bacillus subtilis and Serratia spp., were tested either alone or as a mixture for suppression of onion neck rot and black mould diseases under field conditions. Samples were taken for determination of total phenols, cartenoides and flavonoids. The average of disease reduction was 18.75 % for mixture compared to 47.0% in the non-treated control. In addition to disease suppression, treatment with a mixture of the antagonists promoted plant growth in terms of increased bulb yield. The mixture of the tested bioagents was more effective in reducing the disease and promoting both plant growth and bulb yield compared with using each of them alone. The result indicated the biocontrol efficacy and storage stabilities were not increased with the enhancement of the concentration of biocontrol agents. Treatment the onion bulb with the bioagents increased cartenoides in mixture treatment and the fungicide Folucolon compared with the control and another tested treatment. Treatment onion bulb with any of the tested bioagents or their mixture decreased total phenols compared with the fungicide Folucolon and control in the two concentrations (10 8 CFU/ml and10 9 CFU/ml). On the other hand, they also increased flavonoids compared with control and the fungicide Folucolon in the two concentrations.