Cárie dentária; Fluoretos; Odontologia preventi va. Dental caries; Fluorides; Preventi ve denti stry.

Objective: To perform chemical analysis and to evaluate the anti-biofilm and hemolytic effect of the essential oil of Cymbopogon citratus. Material and Methods: Gaseous chromatography coupled to mass spectrometer was performed for chemical characterization of the essential oil. To verify the antimicrobial action, the Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and Minimum Fungicidal Concentration (MFC) were determined. From MIC, MBC and MFC data, concentrations were established to verify the antibiofilm effect and for the hemolysis test on human erythrocytes. A multispecies biofilm was developed in vitro and mouthwash applications were simulated to determine the inhibition of biofilm formation or its removal. Results were analyzed through ANOVA statistical test, complemented by the Tukey test, considering a significance level of 5%. Results: The major component of the essential oil is citral. MIC verified for Streptococcus mutans was 1mg / mL, while for Candida albicans, it was 125 µg/mL, presenting microbicidal effect for both microorganisms tested. The essential oil was able to inhibit biofilm formation (p<0.001), presenting non-toxic hemolysis percentage in concentration below 500 µg/mL. Conclusion: The essential oil of Cymbopogon citratus is antimicrobial, antibiofilm and non-toxic to human erythrocytes, representing a natural product with potential for use in Dentistry.

Aim. To evaluate the antifungal potential of the essential oil of Cymbopogon citratus by determining the Minimum Inhibitory Concentration (MIC) and the Minimum Fungicidal Concentration (MFC) for Candida albicans (ATCC 90029), Candida albicans (CBS 562), Candida tropicalis (ATCC 705) and Candida tropicalis strains (CBS 94), as well as to analyze the possible mechanism of action of the oil through the addition of sorbitol to the culture medium. Methods. For the MIC determination, inocula were previously adjusted through spectrophotometry and 100μL were added to the wells of plates already containing the culture medium and 100μL of the serial dilutions of the oil, incubating them in aerobiosis for 24 hours, with subsequent staining by 1% TCT. For the MFC, 50μL of the supernatant from the MIC assay wells were dripped onto Petri dishes and incubated in aerobiosis for 24 hours. Tests were performed in triplicate and data analysed by descriptive statistics. Results. It was determined that the MIC for C. albicans was 125 μg/mL while MIC for C. tropicalis was 250 μg/mL, with the essential oil presenting fungicidal effect for both analyzed yeasts. Conclusion. The essential oil of Cymbopogon citratus does not act at the cellular wall level and demonstrated an antimicrobial effect on Candida albicans and Candida tropicalis, therefore acting as a fungicide.

Introduction: Dental caries remains one of the most prevalent oral diseases, which encourages the development of new therapeutic alternatives aiming at its control. Objective: To evaluate the anti-adherent effects of phytotherapic agents against Streptococcus mutans (ATCC 25175). Material and Methods: The tinctures of Plectrantus amboinicus, Conyza bonariensis and Cymbopogon citratus, obtained in compounding pharmacy, were used in this study for determination of their Minimum Inhibitory Concentration of Adherence (MICA). Chlorhexidine (0.12%) and grain alcohol were used as the positive and negative controls, respectively. In order to determine the MICA, S. mutans was inoculated in Mueller Hinton broth supplemented with 10% sucrose in the presence of different concentrations of the products. Then the test tubes were placed in an incubator with a 30° tilt angle under microaerophilic atmosphere for 48 h. This procedure allowed for the development of an adhesive pellicle onto the glass. Subsequently, the tubes were washed with phosphate buffer solution and dyed with plaque disclosing solution (Replac). All tests were performed in duplicate and the data were analyzed using descriptive statistics. Results: The MICA of the tinctures that prevented S. mutans adherence to the glass tubes was 200 µg/mL. Conclusion: The tinctures proved to be effective in preventing S. mutans adherence to glass tubes.

Pacientes com necessidades especiais (PNE) necessitam de atendimento especializado e os profissionais de saúde precisam ter esse conhecimento ainda na graduação. Com isso, é de considerável importância existir a disciplina de PNE nos cursos de Odontologia. Objetivo: Apresentar o panorama da abordagem da disciplina de PNE nos currículos dos cursos de Odontologia, no Nordeste do Brasil. Metodologia: Este estudo é do tipo documental quantitativo com análise descritiva onde por meio de uma busca realizada na internet no site do MEC e das 101 Instituições de Ensino Superior (IES) com o curso de odontologia no Nordeste, onde no site de todas as IES cadastradas foi buscado suas grades curriculares e ementas e analisadas de forma quantitativa. As instituições foram avaliadas sobre: a presença ou ausência de disciplina exclusiva para PNE apresentado em seu currículo; a forma de oferta; e as disciplinas afins. Resultados: A maior parte dos currículos das instituições estava disponível para acesso livre. Verificou-se que 75,2% das IES do Nordeste disponibilizam matriz e/ou ementa via internet. Observou-se que dos currículos disponíveis,44,7% das instituições apresentam componente específico de PNE. Desta parcela, 91,1% ofereciam-na como obrigatória e 8,8% como eletivas e 21% das IES com matriz/ementa disponível apresentam disciplinas afins. Conclusões: Menos da metade das IES do Nordeste brasileiro oferecem uma disciplina no curso de Odontologia exclusiva para PNE e m relação ao modo de ofertar o componente, predominou o obrigatório. Descritores: Deficiência. Instituições de Ensino Superior. Odontologia.