Radical hysterectomy and vaginectomy with sigmoid vaginoplasty was a reasonable option for patients with stage I vaginal carcinoma who wished to retain sexual function after surgery.

Objective of the study is to find the association between p21 rs1059234 polymorphisms and endometrial cancer in Han women from Northeast China. Genotyping of rs1059234 in the p21 gene was performed by sequencing and PCR-restriction fragment length polymorphism (PCR-RFLP) of peripheral blood samples in 263 endometrial cancer patients (case group) and 315 healthy subjects (control group). C allele frequency of rs1059234 was significantly increased in patients of the case group when compared with that in the control group (P = 0.039). The CC genotype frequency of rs1059234 was also significantly increased in patients of case group (0.335, vs. 0.219, P = 0.045). CC genotype increased the risk of endometrial cancer (OR = 1.589, 95%CI: 1.010-2.502). However, after corrected with regression analysis, the CC genotype did not increase the risk of endometrial cancer (OR = 1.623, 95% CI: 0.720-3.659, P = 0.243). Our study demonstrates that in Han women from Northeast China, there is no association between rs1059234 polymorphism of p21 gene and risk of endometrial cancer.

No abstract

The aim of the study was to investigate the inhibitory effects of RNA interference on XIAP gene expression of human endometrial carcinoma RL95-2 cell and the cell apoptosis. Specific small interference RNA (siRNA) of XIAP was designed and composed. Transfection of siRNA was conducted in endometrial carcinoma cell line RL95-2. The XIAP gene mRNA was assessed by real-time PCR and the change of XIAP protein was assessed with Western Blotting. The cell proliferation and apoptosis was assessed by MTT and flow cytometry methods. After transfection of siRNA specifically targeting XIAP, the relative fold of mRNA transfection in the specific transfection group was (0.04 ± 0.06) and the relative protein expression was (0.590 ± 0.178), which was significantly decreased when compared with the control group (P < 0.05); the cell growth inhibition rate in the transfection group was (47.86 ± 4.46)%, which was significantly increased when compared to the control group (P < 0.05). In vitro experiment showed that synthetic siRNA could effectively inhibit the transfection and expression of XIAP gene of human endometrial carcinoma RL95-2 cell at the mRNA level and protein level, thus significantly promote the apoptosis of endometrial carcinoma cell. The mechanisms involved in the apoptosis still require further investigation.