Objectives: This study aimed to investigate the knowledge and attitude of the public in Saudi Arabia toward the concept of surface decontamination during the COVID-19 pandemic.Methods: A cross-sectional, web-based study was conducted over six months, from February 2021 to July 2021. We included adult Saudi and non-Saudi males and females living in Riyadh, Saudi Arabia.Results: Six hundred and twenty-six responses from Saudi (92.7%) and non-Saudi (7.3%) participants with a median age of 24 years and interquartile range (IQR) of 21-29 years were received. Regarding knowledge level, 32.10% of the participants had good knowledge of respiratory virus transmission, and only 3.4% had good knowledge of decontamination products. Overall, 58.1% of the participants had a positive attitude toward decontamination products, and 28% had a negative attitude. Older participants, females, and participants who received their information from the Ministry of Health had higher odds of having a positive attitude toward disinfectant (OR = 1.022, 95% CI: 1.004 to 1.039, p = 0.013), (OR = 3.05, 95% CI: 2.08 to 4.47, p < 0.001), and (OR = 2.95, 95% CI: 1.44 to 6.05, p = 0.003), respectively. Conclusion:The current evidence suggests that the knowledge in the general population of Saudi Arabia is low regarding the transmission of COVID-19 infection and disinfectant products. The prevalence of using decontamination products and attitude toward it is average. Continuous awareness campaigns are required to increase the public's awareness toward such products to change the population's attitude and practice, improve the prevention, and reduce the spread of the infection and its related misconception.