This study was conducted to evaluate the influence of ionizing irradiation supplement diet with replacement of antibiotics in poultry at the level of 5KGy (1Gy= 1 joule/kg). In our study various parameters were analyzed including feed intake, FCR, dressing percentage, weight gain, mortality percentage, weight of goblet and TFT thyroid function test were performed. In this experiment total 9 hundred broiler chicks were purchased from local market and randomly divided into two groups 1 control and 1 irradiated. These groups were further distributed in two more replications with 50 birds per replication using the random treatment design. The findings our study showed that there was no significant influence of feed intake was recorded with irradiation. The results for feed intake of total chick was observed 2652.33±59.45 and 2625.78±16.31g was observed for control and irradiated group, respectively. The results for weight gain was observed significantly higher (P≥0.05) in 1 st and 2 nd week of study in control group, while the results for weight gain was observed significantly higher in (P≥0.05) irradiated group as compared with control group during the 3 rd and 4 th week of experiment. The results for overall weight gain for each broiler was recorded significantly higher (P≥0.05) in irradiated group as compared with control group 1349.00±12.59 and 1286.68±21.08g, respectively. In this study the results for feed conversion ratio was observed higher in irradiated group as compared with control group 1.95±0.02 and 2.06±0.05g, respectively. Whereas the overall results for feed conversion ratio was recorded significantly lower (P≥0.05) in irradiated group as compared with control group. The results for feed conversion ratio was observed 5.33% reduced in irradiated group as compared with control one. There was no significant difference was observed in 1 st , 2 nd , 3 rd and 4 th week of study. There was no significant difference was recorded for the results of dressing % in control and irradiated group. The findings for mortality % was observed significantly higher in (P≥0.05) in control group as compared with the irradiated group. It has also been seen that there was no any significant difference was recorded in Giblets weight and serum triiodothyronine T3, T4 and TSH of both control and irradiated groups. It has been concluded from the present study that irradiated feed can be used at the place of antibiotics with 5KGy level to decrease the mortality % for achieving maximum body weight, body growth and better FCR with less public health problems.