Pharmacists play an important role in detection, assessment, understanding, and prevention of adverse drug reactions (ADRs) or any other drug related problems. The purpose of this study was to determine the relationship between knowledge, perceptions, and attitudes of pharmacists in the field of service in the city of Padang-Mentawai regarding pharmacovigilance. This research is an analytical descriptive study with a cross-sectional approach carried out from March to June 2021. Data collection was carried out by filling out online questionnaires using google Forms. The results showed that one hundred percent of respondents had high knowledge, a positive perception of 97.5%, and a good attitude of 94.9%. There is a significant difference in average knowledge based on pharmacists' experience participating in scientific activities on pharmacovigilance; there is a significant difference in the average perception based on the characteristics of age and place of practice of pharmacists. Based on the correlation test, there is a significant relationship between knowledge and perception of pharmacists and pharmacists' knowledge and attitudes. In conclusion, there is a significant relationship between knowledge and perceptions and attitudes of pharmacists in the field of service in the city of Padang-Mentawai towards pharmacovigilance