Background: There is a growing need for contemporary application of microscopic techniques in laboratory practice. Moreover, the oil immersion microscopy is rarely done because of its intricacy. As such concerned scientists need to be abreast of the step-by-step procedures for preparing a biological specimen on the whole as well as method for increasing the resolving power of a microscope. Aim: To prepare slides from various organ systems with photomicrographs of different resolutions. Materials and Methods: Tissue samples were sourced from laboratory Wistar rats ( Rattus norvegicus). Other research items included histopathology lab reagents as well as equipment including embedding mould, water bath, oven and rotary microtome. Standard method of paraffin embedding was used with some modification and sections stained with hematoxylin (Harris) and eosin. Images were prepared using a photomicroscope connected to a computer interface. Results: 500 pieces of microscope slides encompassing 20 different organ/tissue samples, with each mounted on 25 glass slides, while the photomicrographs taken at low (x100), high (x400) and higher (x1000) power magnifications were produced from the various specimens and saved on a disk. Conclusion: The outcome demonstrates the adequacy of the histological protocol used to guide researchers in related fields.

The relevance of histology slides in microscopy cannot be overemphasized. However, a major challenge to many scientists in the field stems from paucity of published data to nearly lack of access to a comprehensive technical guide to prepare tissue samples as needed for practical demonstrations, reviews or diagnostic purposes. The current research project was carried out to produce histology glass slides and photomicrographs, as well as to document the step-by-step procedures for model by concerned practitioners and scientists. The standard procedures for paraffin method of histological preparation were employed. This involves sample fixation, dehydration, clearing, impregnation, embedding, section-cutting, staining and mounting. Harris hematoxylin and eosin were used for section staining. Tissue specimens were sourced from laboratory rats (Rattus norvegicus). Other materials used included reagents- formalin, alcohol, xylene, distyrene plasticizer xylene (DPX) as well as instruments/equipment- embedding mould, water bath, oven and rotary microtome. Photomicrographs were produced using digital camera attached to light microscope that is connected to a computer interface. 500 pieces set of slides comprising 20 different organ specimens was produced as samples, with each specimen mounted on 25 glass slides of either longitudinal or transverse sections. 40 digital images taken at low and high magnifications were also generated from the various specimens and subsequently stored on a disk. This project occupies a special place in practice due to the clarity about the techniques and the good quality of the slide series obtained which both contribute to knowledge.