While the valvulopathic effects of serotonin (5HT) and angiotensin-II (Ang-II) individually are known, it was not clear how 5HT and Ang-II might interact, specifically in the context of the mechanobiological responses due to altered valve mechanics potentiated by these molecules. In this context, the hypothesis of this study was that increased serotonin levels would result in accelerated progression toward disease in the presence of angiotensin-II-induced hypertension. C57/BL6 J mice were divided into four groups and subcutaneously implanted with osmotic pumps containing: PBS (control), 5HT (2.5 ng/kg/min), Ang-II (400 ng/kg/min), and 5HT + Ang-II (combination). Blood pressure was monitored using the tail cuff method. Echocardiography was performed on the mice before surgery and every week thereafter to assess ejection fraction. After three weeks, the mice were sacrificed and their hearts excised, embedded and sectioned for analysis of the aortic valves via histology and immunohistochemistry. In separate experiments, porcine valve interstitial cells (VICs) were directly stimulated with 5HT (10 M), Ang-II (100 nM) or both and assayed for cellular contractility, cytoskeletal organization and collagen remodeling. After three weeks, average systolic blood pressure was significantly increased in the 5HT, Ang-II and combination groups compared to control. Echocardiographic analysis demonstrated significantly reduced ejection fraction in Ang-II and the combination groups. H&E staining demonstrated thicker leaflets in the combination groups, suggesting a more aggressive remodeling process. Picrosirius red staining and image analysis suggested that the Ang-II and combination groups had the largest proportion of thicker collagen fibers. VIC orientation, cellular contractility and collagen gene expression was highest for the 5HT + Ang-II combination treatment compared to all other groups. Overall, our results suggest that 5HT and Ang-II interact to result in significantly detrimental alteration of function and remodeling in the valve.

Purpose As library budgets continue to constrict, librarians will need to become more familiar with comparing database host platforms. This paper aims to compare Inspec on Elsevier’s Engineering Village (EV) and Clarivate’s Web of Science (WOS) from a novice user experience. The main objectives are to identify some R1 institutions that subscribe to Inspec and highlight some of the key differences between the two platforms. Design/methodology/approach Information on Inspec was gathered from various sources such as the home website, IET, and the host platform websites of Elsevier and Clarivate Analytics. Additional evidence was also collected from brochures and guides to help illustrate some of the main features and differences that novice users would be familiar with. Findings Most institutions subscribe to Inspec via the EV platform. Results from the study conclude that EV was the choice over WOS for hosting Inspec owing to a more user-friendly interface, potential lower cost and faster platform updates in response to meeting user needs. Research limitations/implications As database platforms change over time, feature areas such as content, interface and features remain important for information professionals and librarians to stay current with those changes. Also, this work can help librarians with understanding the planning and developing of a process for comparing identical or similar databases on different platforms. Originality/value Much of the literature focuses on the unfamiliar details and not so much on the novice user. This paper provides a unique perspective in how a novice user would prefer the attributes of one host platform over the other. Additionally, the same review criteria can be applied in other subjects and disciplines.