Diare merupakan penyakit gangguan sistem pencernaan yang potensial dan mewabah. Salah satu cara untuk mengurangi terjadinya kasus diare adalah dengan proses mencuci dan menyimpan  alat makan dengan benar. Pencucian alat makan setelah digunakan masih memungkinkan adanya kandungan bakteri seperti Escherichia coli. Adanya bakteri  pada alat makan tidak sesuai dengan Keputusan Menteri Kesehatan RI Nomor 1098/Menkes/SK/VII/2003 karena pada peralatan makan yang kontak langsung dengan makanan yang siap disajikan angka kumannya tidak boleh melebihi 100/cm2 permukaan alat makan, sedangkan untuk bakteri Escherichia coli harus 0 kuman/cm2 permukaan alat makan. Tujuan penelitian ini untuk mengetahui penurunan total bakteri pada alat makan setelah dilakukan pencucian dengan sabun pencuci piring yang ditambah dengan  1000 mg/lt , 2000 mg/lt ,3000 mg/lt dan 4000 mg/lt ekstrak etanol Mirabilis Jalapa . Metode penelitian yang digunakan adalah Pre-test Post-test Design. Hasil penelitian dengan Uji T berpasangan, didapatkan nilai p value = 0,001, pada α= 5% terdapat  perbedaan yang signifikan antara penurunan  jumlah total bakteri pada alat  makan sebelum dan sesudah pencucian dengan sabun Mirabilis Jalapa  dengan variasi konsentrasi 1000 ppm, 2000 ppm ,3000 ppm dan 4000 ppm. Dari hasil penelitian dapat disimpulkan bahwa terjadi penurunan jumlah bakteri pada alat makan sesudah dilakukan pencucian dengan menggunakan sabun yang ditambah ekstrak  Mirabilis Jalapa   2000 ppm dengan rata-rata penurunan 99,9%.

Water is one of the humans' basic needs that are essential in daily lives. The water use related to the production of the food processing industry must at least meet the quality standards required for drinking water. The Production process water is obtained from the artesian well that has been treated with a physical treatment process. The microbiological examination result of total Coliform is 8.6 MPN/100 ml meaning that it does not meet the quality standard requirements, which is 0 MPN/100 ml (The Minister of Health Regulation Number 492 of 2010, concerning Drinking Water Quality Requirements), it is necessary to treat the production process water using a Melt Blown Filter (MBF) and UV-C rays. The purpose of this study was to determine the differences in the variation of the 1, 3, and 5-micron of Melt Blown Filter cartridge 10" in reducing the amount of total Coliform, as well as the water hardness and their effect on disinfection using UV-C rays. The type of research used is experimental research with a pretest-posttest without control research design. There is a reduction in the average total Coliform after the treatment using a Melt Blown Filter, with a result of 1, 3, and 5-micron as 2.95 MPN /100ml, 3.61 MPB/100 ml, and 7.31 MPN/100 ml with a percentage reduction of 100%, 94.5%, and 82.4%, respectively. The average total Coliform using the Melt Blown Filter equipped with the UV-C rays treatment resulted in a reduction of 1, 3, and 5-micron as 2.95 MPN/100 ml, 3.95 MPN/100 ml, 8.88 MPN/100 ml respectively, with a reduction percentage of 100%, for each treatment. The data analysis for total coliform using the One-Way ANOVA test resulted in a pvalue of 0.001, the effective variation obtained is 1-micron MBF equipped with UV-C rays. The analysis of results pertaining to the water hardness data showed that the use of Melt Blown Filter could not reduce water hardness, but the UV-C rays could still be used accordingly. The data analysis for water hardness was performed using Kruskal-Wallis with a p-value of 0.820, meaning that there are no differences in Melt Blown Filter variation on the results of total water hardness value.