Maximum production of alkaline serine protease by Bacillus alcalophilus subsp. halodurans KP 1239 was achieved after 24 h cultivation, at an initial pH of 7.6, on a medium containing 1.0% sodium citrate, 0.3% yeast extract, and 0.3% KH2PO4. The enzyme was purified to crystalline form from culture broth. The enzyme was most active at 60 degrees C and at pH 11.5. The molecular weight, isoelectric point and sedimentation coefficient in water at 20 degrees C were estimated as 29,000, 8.8 and 3.3S, respectively. The N-terminal amino acid sequence was Ala-Gln-Ser-Val-Pro-Trp-Gly-Ile-Ser-Arg-Val-Gln-Ala-Pro-Ala-Ala- His-Asn-Arg-Gly-. The enzyme shared its antigenic determinants with B. alcalophilus ATCC 21522 serine protease, but not with the subtilisins Carlsberg and BPN'.
Convergent synthesis of dolastatin 15 (1), a cytostatic depsipeptide isolated from the Indian Ocean sea hare, has been described. For construction of the backbone, a single-step condensation of peptide fragment 2 and pyrrolidone fragment 3 was successfully performed using 2-chloro-1,3-dimethyl-2-imidazolinium hexafluorophosphate (CIP) developed by us as an efficient coupling reagent. Coupling of an N-methylamino acid on solid support was first achieved using CIP for the efficient synthesis of peptide fragment 2. The effectiveness of CIP for the coupling of N-methylamino acids in solution and on solid support were clarified by the syntheses of model di-and tripeptides.
Convergent synthesis of (−)-mirabazole C
(1), a tetra thiazoline/thiazole alkaloid isolated from
blue-green alga, has been described. The successive thiazoline rings of
(−)-mirabazole C were formed
by a single-step cyclization mediated by TiCl4 treatment of
tripeptide amide 4. Convergent synthesis
of the key intermediate 33 derived from three
2-methylcysteine residues was first achieved using
a newly developed coupling reagent, 2-chloro-1,3-dimethylimidazolidium
hexafluorophosphate (CIP).
The effectiveness of CIP for the coupling of α,α-dialkyl amino
acids and the reaction pathway of
the activation were clarified by the syntheses of model peptides
containing an α,α-dimethylamino
acid. A practical method of asymmetric synthesis of
2-methylcysteine by alkylation of 2,4-cis-oxazolidinone 23 has also been described.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.