We investigated the shape of a well to trap single cells for the purpose of improving the cell trapping rate after cell seeding and liquid exchange in a cell microarray. The wells having vertical and inclined tapered side‐wall were made using three‐dimensional UV photolithography. Changes in cell trapping rate by the shape of the wells were evaluated by trapping tests with fluorescent microbeads and living cells. As a result of trapping test with living cells, bead residual rate of the microarray with inclined tapered wells is up to 2.9 times higher than that with vertical wells.

No abstract

We investigated the shape of a well to trap single cells for the purpose of improving the cell trapping rate after cell seeding and liquid exchange in a cell microarray. The wells having vertical and inclined tapered side-wall were made using three-dimensional UV photolithography. Changes in cell trapping rate by the shape of the wells were evaluated by trapping tests with fluorescent microbeads and living cells. As a result of trapping test with living cells, bead residual rate of the microarray with inclined tapered wells is up to 2.9 times higher than that with vertical wells. K E Y W O R D S3D photolithography, single cell, well array Electron Comm Jpn. 2018;101:45-50.